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05-1308-AF647 Anti-Ubiquitin, Lys63 Antibody, clone Apu3, Alexa Fluor® 647 Conjugate

Anti-Ubiquitin, Lys63 Antibody, clone Apu3, Alexa Fluor® 647 Conjugate is an antibody against Aniti-Ubiquitin for use in Immunocytochemistry.

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Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: UBB, UBC UniProt Number: P0CG48
05-1308-AF647
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, RbICCRbAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue Number05-1308-AF647
      DescriptionAnti-Ubiquitin, Lys63 Antibody, clone Apu3, Alexa Fluor® 647 Conjugate
      Alternate Names
      • Lys63-linked ubiquitin
      Background InformationPolyubiquitin-C (UniProt P0CG48) is encoded by the UBC gene (Gene ID 7316) in human. Ubiquitin (Ub) is initially produced as a 685-a.a. precursor protein whose C-terminus Val685 is post-translationally removed, followed by cleavages between a.a. 77 and 152, a.a. 153 and 228, a.a. 229 and 304,a.a. 305 and 380, a.a. 381 and 456, a.a. 457 and 532, a.a. 533 and 608, a.a. 609 and 684 to yield nine identical 76-amino acid ubiquitin (also known as high mobility group nonhistone protein HMG-20) molecules. Ub is involved in one of the most common post-translational modifications (PTMs) of cellular proteins, where Ub is linked covalently via its carboxyl terminus to usually Lys residues in target proteins. A given target Lys residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyubiquitin chain, ubiquitin molecules can be linked through one of the seven ubiquitin Lys residues (K6, K11, K27, K29, K33, K48, and K63) or through the Ub N-terminus Met1 residue (which generates linear chains). An Ub chain with a single linkage type is referred to as homotypic, whereas an heterotypic chain contains mixed linkages within the same chain, which can lead to branched/forked (as a result of one Ub linked to two or more Ubs) or non-branched Ub chains. Lys48-linked chains mark proteins for proteasomal degradation, while Lys63-linked chains regulate the activity/function of cellular proteins.
      Product Information
      FormatAlexaFluor®647
      PresentationPurified rabbit monoclonal IgG antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Ubiquitin, Lys63 Antibody, clone Apu3, Alexa Fluor® 647 Conjugate is an antibody against Aniti-Ubiquitin for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Lys63-linked ubiquitin in HUVECs, A431 and NIH/3T3 cells.
      The unconjugated antibody (Cat. No. 05-1308) is shown to be suitable also for Western blotting, peptide inhibition assay, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications.
      Biological Information
      ImmunogenImmunogen used was human di-ubiquitin linked at Lys63.
      EpitopeLys63 ubiquitination site.
      CloneApu3
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityRecognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • UBB
      • UBC
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular WeightVariable
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Lys63-linked ubiquitin in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      05-1308-AF647 04055977314588