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05-623-25UG Anti-RNA polymerase II Antibody, clone CTD4H8

Anti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors UniProt Number: P24928
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05-623-25UG
25 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Yeast (S. cerevisiae)WB, ChIP-seqMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-623-25UG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-RNA polymerase II Antibody, clone CTD4H8
      Background InformationRNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
      References
      Product Information
      FormatPurified
      Control
      • A431 nuclear extract.
      PresentationPurified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
      Key Applications
      • Western Blotting
      • ChIP-seq
      Biological Information
      ImmunogenPeptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
      CloneCTD4H8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThis antibody recognizes Phospho- and non-phospho-RNA polymerase II.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Yeast (S. cerevisiae)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G Chromatography
      UniProt Number
      Molecular Weight210-220 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on HeLa nuclear extracts.

      Western Blot Analysis:
      0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-623-25UG 04054839350191

      Documentation

      Anti-RNA polymerase II Antibody, clone CTD4H8 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-RNA polymerase II Antibody, clone CTD4H8 Certificates of Analysis

      TitleLot Number
      Anti-RNA polymerase II, clone CTD4H8 - 3174755 3174755
      Anti-RNA polymerase II, clone CTD4H8 - 3230980 3230980
      Anti-RNA polymerase II, clone CTD4H8 - 3286829 3286829
      Anti-RNA polymerase II, clone CTD4H8 - 3536051 3536051
      Anti-RNA polymerase II, clone CTD4H8 - 3710691 3710691
      Anti-RNA polymerase II, clone CTD4H8 - 3840860 3840860
      Anti-RNA polymerase II, clone CTD4H8 - 4035810 4035810
      Anti-RNA polymerase II, clone CTD4H8 - 4102570 4102570
      Anti-RNA polymerase II, clone CTD4H8 - 4163017 4163017