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MABC580 Anti-Clathrin Heavy Chain Antibody, clone 6F10.1

Anti-Clathrin Heavy Chain Antibody, clone 6F10.1 is an antibody against Clathrin Heavy Chain for use in Western Blotting, Flow Cytometry, Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: CLTC, CLH17, CLTCL2, KIAA0034 UniProt Number: Q00610
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MABC580
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWB, FC, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC580
      DescriptionAnti-Clathrin Heavy Chain Antibody, clone 6F10.1
      Alternate Names
      • Clathrin heavy chain 1
      • Clathrin Heavy Chain
      • Clathrin heavy chain on chromosome 17
      • CLH-17
      Background InformationClathrin heavy chain 1 (UniProt Q00610; also known as Clathrin heavy chain on chromosome 17, Clathrin heavy polypeptide (Hc), Clathrin heavy polypeptide-like 2, CLH-17) is encoded by the CLTC (also known as CHC, CLH17, CLTCL2, KIAA0034) gene in human (Entrez Gene ID 1213). Clathrin is the major protein component of the polyhedral coat of coated pits and vesicles. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of various macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Clathrin Heavy Chain Antibody, clone 6F10.1 is an antibody against Clathrin Heavy Chain for use in Western Blotting, Flow Cytometry, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      • Immunocytochemistry
      Application NotesFlow Cytometry Analysis: 1.5 µg of this antibody from a representative lot detected Clathrin Heavy Chain in 1X10E6 Jurkat cells.
      Immunocytochemistry Analysis: 25.0 µg/mL from a representative lot detected Clathrin Heavy Chain in HeLa, A431, HUVEC, and NIH/3T3 cells.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Clathrin Heavy Chain near the C-terminus.
      Clone6F10.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CLTC
      • CLH17
      • CLTCL2
      • KIAA0034
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~190 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Clathrin Heavy Chain in 10 µg of PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC580 04055977166620

      Documentation

      Anti-Clathrin Heavy Chain Antibody, clone 6F10.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Clathrin Heavy Chain Antibody, clone 6F10.1 Certificates of Analysis

      TitleLot Number
      Anti-Clathrin Heavy Chain, -Q2569081 Q2569081
      Anti-Clathrin Heavy Chain, clone 6F10.1 - 3426770 3426770
      Anti-Clathrin Heavy Chain, clone 6F10.1 - 3736706 3736706
      Anti-Clathrin Heavy Chain, clone 6F10.1 Monoclonal Antibody 3065503

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies