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GR08L Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14)

This Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) is validated for use in FC, IF, IP, Immunoblotting for the detection of Transferrin Receptor (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TR, Anti-CD71

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GR08L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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GR08L-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor in the plasma membrane in proliferating cells.
      Catalogue NumberGR08L
      Brand Family Calbiochem®
      SynonymsAnti-TR, Anti-CD71
      References
      ReferencesSchneider, C., et al., 1982. J. Biol. Chem. 257, 8516.
      Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
      Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem. 49, 357.
      Omary, M.B., et al. 1980. Nature 286, 888.
      Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlMost cell lines or placental tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Trowbridge, I.S. and Omary, M.B., 1981. Proc. Natl. Acad. Sci. USA 3039.
      Key Applications Flow Cytometry
      Immunofluorescence
      Immunoprecipitation
      Not Western Blot
      Application NotesFlow Cytometry (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Immunoblotting (not recommended)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenK562 cells
      ImmunogenHuman
      CloneT56/14
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GR08L-100UG 04055977228199

      Documentation

      Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14) Certificates of Analysis

      TitleLot Number
      GR08L

      References

      Reference overview
      Schneider, C., et al., 1982. J. Biol. Chem. 257, 8516.
      Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
      Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem. 49, 357.
      Omary, M.B., et al. 1980. Nature 286, 888.
      Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-October-2010 RFH
      SynonymsAnti-TR, Anti-CD71
      ApplicationFlow Cytometry (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Immunoblotting (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with S194/5XXO.BU.1 mouse myeloma cells (see application references). Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor protein.
      BackgroundTransferrin is the major iron binding protein in human plasma and is bound by a specific cell surface receptor during transport of iron into various tissues. The human transferrin receptor was originally isolated from placenta and shown to contain a subunit with an apparent molecular weight of 90,000. Further structural characterization of the receptor revealed that it resides in the plasma membrane as a 180,000 dimer, has a pI of 5.2, is phosphorylated predominantly on serine residues and contains both complex and high mannose oligosaccharide chains. Transferrin receptor is considered to be a marker for proliferating cells and its levels can be drastically reduced by agents which cause differentiation of undifferentiated cells such as HL-60 cells. Since many of the enzymes of metabolically active, proliferating cells require iron to catalyze redox reactions it is not surprising that the transferrin receptor would be a marker of proliferation. Further support for this hypothesis has been obtained by using antibodies to block transferrin binding and cause the accumulation of cells in S-phase, presumably due to interference with normal iron uptake and energy metabolism. Thus, monoclonal antibodies recognizing the transferrin receptor are not only valuable tools for studying iron metabolism, but may also be useful for monitoring and possible therapy of diseases involving highly proliferative cells.
      HostMouse
      Immunogen speciesHuman
      ImmunogenK562 cells
      CloneT56/14
      IsotypeIgG₁
      Specieshuman
      Positive controlMost cell lines or placental tissue
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesSchneider, C., et al., 1982. J. Biol. Chem. 257, 8516.
      Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
      Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem. 49, 357.
      Omary, M.B., et al. 1980. Nature 286, 888.
      Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
      Application referencesOriginal Clone Trowbridge, I.S. and Omary, M.B., 1981. Proc. Natl. Acad. Sci. USA 3039.