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07-473-AF647 Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 647 Conjugate

This Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
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07-473-AF647
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICCRbAlexaFluor®647Polyclonal Antibody
      Description
      Catalogue Number07-473-AF647
      DescriptionAnti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 647 Conjugate
      Alternate Names
      • H3K4me3
      • Histone H3 (trimethyl Lys4)
      • H3 histone family, member T
      • Histone 3, H3
      • Histone cluster 3, H3
      Background InformationHistone H3 is one of the five main histone proteins that are involved in the structure of chromatin in eukaryotic cells. Histone H3 features a main globular domain and a long N-terminal tail. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 is specifically phosphorylated during both mitosis and meiosis in patterns that are specifically coordinated in space and time. These phosphorylations are initiated at different phases of the cell division. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified rabbit conjugate antibody in buffer containing PBS with 15 mg/ml BSA and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 647 Conjugate is validated for use in Immunocytochemistry for the detection of trimethyl-Histone H3.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected trimethyl-Histone H3 (Lys4) in NIH/3T3 cells.
      The unconjugated version of this antibody has been shown to work in ChIP, DB, WB, ChIP-seq.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues surrounding and including trimethlyated Lys 4 of Histone H3.
      EpitopeTrimethylated (Lys4)
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Vertebrates based on 100% sequence homology. Expected to react with most other species based on extreme conserved homology among species.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Modifications
      • Methylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~16 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys4) in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-473-AF647 04055977182026

      Documentation

      Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 647 Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-trimethyl-Histone H3 (Lys4) Antibody, Alexa Fluor® 647 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 647 - 3948897 3948897
      Anti-trimethyl-Histone H3 (Lys4), Alexa Fluor® 647 -Q2586398 Q2586398

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.