Millipore Sigma Vibrant Logo

HTS190RTA Ready-to-Assay™ NK3 Tachykinin Receptor Frozen Cells

Human NK3 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_001059
HTS190RTA
1 kit  
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Special Offers

      This product requires a User Agreement (Label License). Please see product datasheet for further information

      Key Spec Table

      SpeciesHost CellsProtein Target
      Human Chem-1NK3
      Description
      Catalogue NumberHTS190RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ NK3 Tachykinin Receptor Frozen Cells
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      NK3 is one of three mammalian G-protein coupled receptors subtypes (NK1, NK2, NK3) that mediate the biological effects of the tachykinin peptide family. Neurokinin B is the tachykinin family peptide with the highest affinity to NK3R, but it still induces a response with NK1, and NK2 with lower potency (Pennefather et al., 2004; Regoli et al., 1994; Maggi et al., 1993). NK3, like the other tachykinin receptors, signals through Gq to stimulate intracellular calcium (Pinnock et al., 1994). Mammalian tachykinins are present in the brain and gut. NK3 receptor antagonists reduce intestinal motility and nociception in animal models of gastrointestinal disorders, and may be effective in irritable bowel syndrome (Sanger 2004). Millipore’s cloned human NK3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant NK3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to enhance coupling the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between NK3 and its ligands.
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Detection methodFluorescent
      Quality LevelMQ300
      Applications
      ApplicationHuman NK3 GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      Entrez Gene Number
      GPCR ClassA
      Host CellsChem-1
      Protein TargetNK3
      Target Sub-FamilyTackykinin /neurokinin
      Cell Line Type
      • GPCR Cell Lines
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size1 kit
      Global Trade Item Number
      Catalogue Number GTIN
      HTS190RTA 04053252555336