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05-1059 Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal

Anti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases, Inflammation & Autoimmune Mechanisms Gene Symbol: P38B, P38BETA2, PRKM11, SAPK2 , SAPK2B, p38-2, p38Beta, p38b UniProt Number: Q15759
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05-1059
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, VrtWB, IFRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number05-1059
      DescriptionAnti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8, rabbit monoclonal
      Alternate Names
      • CSAID-binding protein
      • Csaids binding protein
      • Cytokine suppressive anti-inflammatory drug-binding protein
      • MAP kinase MXI2
      • MAP kinase p38 alpha
      • MAX-interacting protein 2
      • Mitogen-activated protein kinase p38 alpha
      • cytokine suppressive anti-inflammatory drug binding protein
      • mitogen-activated protein kinase 14
      • p38 MAP kinase
      • p38 mitogen activated protein kinase
      • p38alpha Exip
      • stress-activated protein kinase 2A
      Background InformationThe p38 family refers to the SAPK2/3 Kinases (Stess-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.
      Product Information
      FormatCulture Supernatant
      PresentationRabbit monoclonal antibody in culture supernatant containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-p38α(Thr180/Tyr182) Antibody, clone 8.78.8 detects level of phospho-p38α(Thr180/Tyr182) & has been published & validated for use in WB & IF.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids surrounding and including phosphorylated Thr180/Tyr182 of human p38α.
      EpitopePhosphorylated Thr180/Tyr182
      Clone8.78.8
      HostRabbit
      SpecificityThis antibody detects p38α when phosphorylated on Thr180/Tyr182.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Vertebrates
      Species Reactivity NoteHuman and Mouse tested. Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]
      Gene Symbol
      • P38B
      • P38BETA2
      • PRKM11
      • SAPK2
      • SAPK2B
      • p38-2
      • p38Beta
      • p38b
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      COFACTOR: Magnesium By similarity.
      ENZYME REGULATION: Activated by phosphorylation on threonine and tyrosine by MKK6. Inhibited by pyridinyl-imidazole related compounds.
      TISSUE SPECIFICTY: Highest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas.
      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme Probable.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
      Molecular Weight38 kDa
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on anisomycin-treated HeLa cell lysate.
      Western Blot Analysis: 1:5,000-1:10,000 dilution of this antibody was used to detect p38 phosphorylated on Thr180/Tyr182 in anisomycin-treated HeLa and NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Catalogue Number GTIN
      05-1059 04053252327872