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MABE1016 Anti-pan-ADP-ribose binding reagent

Anti-poly-ADP-ribose binding reagent is a reagent that selectively binds to both mono- and poly- ADP ribose for use in Western Blotting, Immunocytochemistry and Dot Blot.

Anti-pan-ADP-ribose binding reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: General Post-translation Modification
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MABE1016
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormat
      M, HWB, IP, DBE. coliPurified
      Description
      Catalogue NumberMABE1016
      DescriptionAnti-pan-ADP-ribose binding reagent
      Alternate Names
      • pan-ADP-ribose binding reagent
      Background InformationCat. No.MABE1016, Anti-pan-ADP-ribose binding reagent, is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta™(DE3)pLysS strain of E. coli (Cat. No. 70956). Anti-pan-ADP-ribose binding reagent is useful for the affinity detection of both mono- and poly-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis, The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-pan-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified from E. coli by Ni-NTA agarose. Supplied in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10% Glycerol, 10 mM Imidazole, 1 mM PMSF, 1 mM β-Mercaptoethanol, 10% glycerol without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-poly-ADP-ribose binding reagent is a reagent that selectively binds to both mono- and poly- ADP ribose for use in Western Blotting, Immunocytochemistry and Dot Blot.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Dot Blot
      Application NotesDot Blot Specificity Analysis: This reagent detected mono(ADPR) on recombinant PARP3 protein, as well as mono(ADPR) and poly(ADPR) on recombinant PARP1 recombinant protein (Lee Kraus, University of Texas Southwestern Medical Center).

      Immunoprecipitation Analysis: A representative lot of Anti-pan-ADP-ribose binding reagent immunoprecipitated ADP-ribosylated proteins from nuclear extract (Lee Kraus, University of Texas Southwestern).

      Western Blotting Analysis: A representative lot detected auto-ADP-ribosylation activity of PARP1/2/3 and mutants in the presence of NAD+ or various NAD+ analogs (Gibson, B.A., et al. (2016). Science. 353(6294):45-50).

      Western Blotting Analysis: A representative lot detected PARP1-catalyzed NELF-E ADP-ribosylation in cell-free enzymatic reactions as well as ADP-ribosylation of exogenously expressed FLAG-tagged NELF-E in HEK293T cells. PARP inhibitor PJ34 or P-TEFb/CDK9 inhibitor flavopiridol treatment decreased cellular NELF-E ADP-ribosylation level (Gibson, B.A., et al. (2016). Science. 353(6294):45-50).
      Biological Information
      ConcentrationPlease refer to lot specific datasheet.
      HostE. coli
      Specificitypoly(ADP-ribose) and mono(ADP-ribose)
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human. Predicted to react with all species.
      Purification MethodNi-NTA agarose
      Molecular WeightVariable depending on the target proteins and the extend of ADP-ribosylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting on ADP-ribosylated PARP1 and PARP3 recombinant proteins.

      Western Blotting Analysis: This reagent detected mono(ADPR) on recombinant PARP3 protein, as well as mono(ADPR) and poly(ADPR) on recombinant PARP1 protein (Lee Kraus, University of Texas Southwestern Medical Center).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1016 04055977168266

      Documentation

      Anti-pan-ADP-ribose binding reagent SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-pan-ADP-ribose binding reagent Certificates of Analysis

      TitleLot Number
      Anti-pan-ADP-ribose binding reagent 3074935
      Anti-pan-ADP-ribose binding reagent 3772221
      Anti-pan-ADP-ribose binding reagent 2918927
      Anti-pan-ADP-ribose binding reagent 3126639
      Anti-pan-ADP-ribose binding reagent 3013396
      Anti-pan-ADP-ribose binding reagent 3043706
      Anti-pan-ADP-ribose binding reagent 3153240
      Anti-pan-ADP-ribose binding reagent 2958005
      Anti-pan-ADP-ribose binding reagent 2836182
      Anti-pan-ADP-ribose binding reagent 2901597
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      Technical Info

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      White Paper: Further considerations of antibody validation and usage.