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ABT1838 Anti-Claudin-16

This rabbit polyclonal Anti-Claudin-16, Cat. No. ABT1838, is a rabbit polyclonal antibody that detects Claudin-16 and has been tested for use in Immunocytochemistry, Immunofluorescence, and Inhibition/Function studies.

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ABT1838
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABT1838
      DescriptionAnti-Claudin-16
      Alternate Names
      • Paracellin-1
      • PCLN-1
      Background InformationClaudin-16 (UniProt: Q9Y5I7; also known as Paracellin-1, PCLN-1) is encoded by the CDLN1 (also known as PCLN1) gene (Gene ID: 10686) in human. Claudin-16 is a multi-pass membrane protein that span the plasma membrane 4 times, with their N- and C-termini located in the cytosol. It is selectively expressed at tight junctions of renal epithelial cells of the thick ascending limb of loop of Henle s where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of divalent cations. Claudin-16 may form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors. Over 20 different mutation sin CLDN1 gene have been reported that are linked to familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC), a renal Mg2+ and Ca2+ wasting disorder that ultimately leads to progressive kidney failure. (Ref.: Muller D et al. (2006). J. Clin. Endocrinol. Metab. 91(8):3076-3079).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.54 M Tris, 0.2 M Glycine, 250 mM NaCl, pH 7.5 without azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-Claudin-16, Cat. No. ABT1838, is a rabbit polyclonal antibody that detects Claudin-16 and has been tested for use in Immunocytochemistry, Immunofluorescence, and Inhibition/Function studies.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Inhibits Activity/Function
      Application NotesImmunofluorescence Analysis: A representative lot detected Claudin-16 in Immunofluorescence applications (Kausalya, P.J., et. al. (2006). J Clin Invest. 116(4):878-91).

      Inhibits Activity/Function: A representative lot blocked Clathrin-mediated endocytosis in MDCK cells (Muller, D., et. al. (2006). J Clin Endocrinol Metab. 91(8):3076-9).
      Biological Information
      ImmunogenConjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Claudin-16.
      Epitopeextracellular domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Claudin-16 in human and canine cells. It targets an epitope within 15 amino acids from the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      • Canine
      Species Reactivity NoteCanine, Human.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CLDN16
      • PCLN1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight33.84 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in MDCK cells.

      Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected Claudin-16 in Madin-Darby Canine Kidney Epithelial (MDCK) cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT1838 04054839402210

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