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SCT263 BioTracker™ Mitophagy Detection Kit

The SCT263 kit is composed of a mitophagy detection probe and a lysosome probe. The mitophagy dye accumulates in intact mitochondria where it is immobilized by chemical bonding and emits weak fluorescence. Upon mitophagy and fusion with the lysosome, damaged mitochondria increase in fluorescence in tandem with decreased pH.

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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT263
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      DescriptionBioTracker™ Mitophagy Detection Kit
      OverviewTwo-probe kit for detection of mitophagy and confirmation of fusion with the lysosome useful for study of PD, AD, and other diseases where mitochondrial dysfunction and processing is known to be critical.

      SPECTRAL PROPERTIES
      Mitophagy probe:
      λex: 530 nm
      λem: 700 nm
      Lysosome probe:
      λex: 398 nm
      λem: 525 nm


      A 100 uM stock solution of the mitophagy probe is created by dissolving 50 uL DMSO to one tube (5 ug) of mitophagy dye. A typical working solution concentration is 1 nM.
      A 1 mM stock solution of the ysosome dye is created by resuspending the contents of the lysosome dye in 55 uL DMSO. A typical concentration for the working solution is 1 uM.
      Alternate Names
      • mitophagy probe
      • mitophagy live cell kit
      • mitophagy detection
      • mitochondrial degradation probe
      Background InformationMitochondria are cytoplasmic organelles that play a crucial cellular role in the production of energy for cell viability and function. Recently, mitophagy has been shown to be related to Alzheimer's and to Parkinson's diseases and induced by the accumulation of depolarized mitochondria. Mitophagy serves as a specific elimination system in which dysfunctional mitochondria damaged by oxidative stress in DNA are sequestered into the autophagosome, fused to the lysosome, and degraded by digestion.
      References
      Product Information
      FormatLyophilized solid
      Presentationpurple solid (mitophagy probe), yellow solid (lysosome probe)
      Quality LevelMQ100
      Applications
      ApplicationThe SCT263 kit is composed of a mitophagy detection probe and a lysosome probe. The mitophagy dye accumulates in intact mitochondria where it is immobilized by chemical bonding and emits weak fluorescence. Upon mitophagy and fusion with the lysosome, damaged mitochondria increase in fluorescence in tandem with decreased pH.
      Key Applications
      • Mitochondria imaging
      • Cell Based Assays
      • Fluorescence
      Application NotesThis live cell probe mitophagy detection kit includes a probe for mitochondrial degradation (far red) plus a probe to confirm association of degraded mitochondria with the lysosome (green).
      The kit can be used in conjunction with SCT272, BioTracker Red Mitochondria Live Cell Probe.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2 - 8 °C, protected from light.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT263 04065270660503