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SCT276 BioTracker™ Green pH-Independent Autophagy Live Cell Probe

Fluorescence microscopy
Flow cytometry

SCT276
5 nmol  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT276
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      DescriptionBioTracker™ Green pH-Independent Autophagy Live Cell Probe
      OverviewSCT276 is a small fluorescent molecule that detects autophagosomes and autolysosomes by a likely mechanism of incorporation into the autophagosome double membrane during its formation, whereupon it emits fluorescence under hydrophobic conditions. This probe has been noted to frequently colocalize with LC3.

      SPECTRAL CHARACTERISTICS:
      Excitation λ: 450 nm
      Emission λ: 535 nm
      Alternate Names
      • green autophagy probe
      • autophagosome live cell probe
      • green autophagosome probe
      • green AP live cell probe
      Background InformationAutophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, dysfunctional proteins aggregate and are enclosed by a double membrane to form an autophagosome.
      References
      Product Information
      Detection methodFlow cytometry Fluorescence
      FormatLyophilized solid
      PresentationLyophilized solid.
      Quality LevelMQ100
      Applications
      ApplicationFluorescence microscopy
      Flow cytometry
      Key Applications
      • Cell Based Assays
      • Fluorescence
      • Flow Cytometry
      • Fluorescence Activated Cell Sorting (FACS)
      Application NotesSCT276 detects autophagosomes and autolysosomes by the likely mechanism of incorporation into the autophagosome during double membrane formation, and emits green fluorescence under hydrophobic conditions. SCT276 is cell permeable, does not require transfection, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry.


      Add 50 uL DMSO to tube of SCT276 (5 nmol) and pipet to dissolve to make a 0.1 mM stock solution. Dilute with culture medium to 0.1 - 0.5 uM to make working solution.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20 °C, protected from light.
      Packaging Information
      Material Size5 nmol
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT276 04065269218562

      Documentation

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