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MABN2285 Anti-Prion Protein Antibody, clone POM1

Anti-Prion Protein, clone POM1, Cat. No. MABN2285, is a highly specific mouse monoclonal antibody that targets PrP and has been tested in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting..

Anti-Prion Protein Antibody, clone POM1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Prnp, Prn-p, Prp UniProt Number: P04925
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MABN2285
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIF, IHC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2285
      DescriptionAnti-Prion Protein Antibody, clone POM1
      Alternate Names
      • Major prion protein
      • PrP
      • PrP27-30
      • PrP33-35C
      • CD230
      Background InformationMajor prion protein (UniProt: P04925; also known as PrP, PrP27-30, PrP33-35C, CD230) is encoded by the Prnp (also known as Prn-p, Prp) gene (Gene ID: 19122) in murine species. PrP is highly expressed in the brain, lung, kidney and heart and low levels of expression are reported in the liver and spleen. It is found in high quantity in the brain of humans and animals infected with degenerative neurological diseases such as Kuru, Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler syndrome (GSS), scrapie, bovine spongiform encephalopathy (BSE), and transmissible mink encephalopathy (TME). In normal brain it may play a role in neuronal development and synaptic plasticity and may be required for neuronal myelin sheath maintenance and myelin homeostasis through acting as an agonist for ADGRG6 receptor. PrP can exist in either PrPc (c = cellular) or PrPsc (sc = Scrapie) form. PrPc is a glycoprotein normally found inserted in the plasma membrane by a glycosyl phosphatidylinositol (GPI) anchor and can be easily digested by proteases. It is composed mainly of alpha-helices. The PrPsc has more of a beta-sheet type structure and is highly resistant to the action of proteases. The prion proteins possess two N-linked glycosylation sites and may contain over 50 different sugars. The large size of the N-linked sugars enables them to shield two orthogonal faces of the protein almost completely and protect large regions of the protein surface from proteases. The PrPsc is shown to contain the same set of glycans as PrPc, but has a higher proportion of tri- and tetra-antennary sugars. Multiplication of prion protein appears to be slower in vivo than in vitro. It is shown that following proteinase K treatment, PrPc is completely digested, whereas PrPsc is only shortened to a 27-30 kDa fragment. (Ref.: Meisl, G., et al. (2021). Nat. Struct. Mol. Bio. 28(4); 365-372).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Prion Protein, clone POM1, Cat. No. MABN2285, is a highly specific mouse monoclonal antibody that targets PrP and has been tested in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting..
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Prion Protein in Western Blotting applications (Kuffer, A., et. al. (2016). Nature. 536(7617):464-8; Polymenidou, M., et. al. (2008). PLoS One. 3(12):e3872).

      Immunofluorescence Analysis: A representative lot detected Prion Protein in Immunofluorescence applications (Kuffer, A., et. al. (2016). Nature. 536(7617):464-8).

      Immunohistochemistry Analysis: A representative lot detected Prion Protein in Immunohistochemistry applications (Polymenidou, M., et. al. (2008). PLoS One. 3(12):e3872).

      Immunoprecipitation Analysis: A representative lot detected Prion Protein in Immunoprecipitation applications (Polymenidou, M., et. al. (2008). PLoS One. 3(12):e3872).

      ELISA Analysis: A representative lot detected Prion Protein in ELISA applications (Polymenidou, M., et. al. (2008). PLoS One. 3(12):e3872).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 209 amino acids from the internal region of murine major prion protein.
      ClonePOM1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone POM1 detects major prion protein in Tga20 mice brain. It targets an epitope within the C-terminal globular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Prnp
      • Prn-p
      • Prp
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~35 kDa observed; 27.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Brain homogenate from a Tga20 mouse overexpressing Prnp versus brain homogenate from a ZH3/ZH3 control mouse lacking Prnp.

      Western Blotting Analysis: 0.5 µg/mL of this antibody this antibody detected PrP in Brain homogenate from a Tga20 mouse overexpressing Prnp versus brain homogenate from a ZH3/ZH3 control mouse lacking Prnp.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2285 04054839324345

      Documentation

      Anti-Prion Protein Antibody, clone POM1 Certificates of Analysis

      TitleLot Number
      Anti-Prion Protein, clone POM1 - 3693114 3693114
      Anti-Prion Protein, clone POM1 - 3788638 3788638
      Anti-Prion Protein, clone POM1 - 3984677 3984677
      Anti-Prion Protein, clone POM1 - 4008836 4008836
      Anti-Prion Protein, clone POM1 - 4058998 4058998
      Anti-Prion Protein, clone POM1 - 4109617 4109617
      Anti-Prion Protein, clone POM1 Monoclonal Antibody Q2981361
      Anti-Prion Protein, clone POM1 Monoclonal Antibody Q2898863