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MABE78 Anti-MiTF Antibody, clone 3D1.2

Anti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.

Anti-MiTF Antibody, clone 3D1.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Transcription Factors Gene Symbol: MITF, BHLHE32 UniProt Number: O75030
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MABE78
200 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE78
      ReplacesMAB3747
      DescriptionAnti-MiTF Antibody, clone 3D1.2
      Alternate Names
      • Microphthalmia-associated transcription factor
      • Class E basic helix-loop-helix protein 32
      • bHLHe32
      Background InformationMicrophthalmia-associated transcription factor (MiTF) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, and development of mast cells and bone. Mutations in MiTF cause auditory pigmentary syndromes in humans, such as Waardenburg syndrome type II and type IIa, and Tietz syndrome. There are two known isoforms of MiTF, differing by 66 amino acids at the N-terminus. Shorter forms are expressed in melanocytes and run as two bands at 52 kDa and 56 kDa, whereas the longer Mi form runs as a cluster of bands at 60-70 kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain tissue lysate
      PresentationPurified mouse monoclonal IgMκ in buffer containing 0.01M PBS, 0.1% Sodium Azide, pH 7.1.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MiTF Antibody, clone 3D1.2 is an antibody against MiTF for use in Western Blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:100 dilution of this antibody detected MiTF in human melanoma tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human MiTF.
      Clone3D1.2
      HostMouse
      IsotypeIgMκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MITF
      • BHLHE32
      UniProt Number
      Molecular Weight~52 kDa observed. Multiple isoforms between 46-56 kDa are known to exist.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain tissue lysate.

      Western Blot Analysis: A 1:500 dilution of this antibody detected MiTF in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE78 04053252698842

      Documentation

      Anti-MiTF Antibody, clone 3D1.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MiTF Antibody, clone 3D1.2 Certificates of Analysis

      TitleLot Number
      Anti-MiTF, clone 3D1.2 - 2562843 2562843
      Anti-MiTF, clone 3D1.2 - 3270773 3270773
      Anti-MiTF, clone 3D1.2 - 3734543 3734543
      Anti-MiTF, clone 3D1.2 - Q2060583 Q2060583
      Anti-MiTF, clone 3D1.2 -2650908 2650908
      Anti-MiTF, clone 3D1.2 -2712568 2712568
      Anti-MiTF, clone 3D1.2 -2750089 2750089
      Anti-MiTF, clone 3D1.2 -2793645 2793645
      Anti-MiTF, clone 3D1.2 Monoclonal Antibody 3050686
      Anti-MiTF, clone 3D1.2 Monoclonal Antibody 3499348

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies