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MABE222 Anti-JMJD2A Antibody, clone 8B7.1, rabbit monoclonal

This Anti-JMJD2A Antibody, clone 8B7.1 is validated for use in Western Blotting, IHC for the detection of JMJD2A.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: KDM4A, JHDM3A, JMJD2, KIAA0677 UniProt Number: O75164
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MABE222
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE222
      DescriptionAnti-JMJD2A Antibody, clone 8B7.1, rabbit monoclonal
      Alternate Names
      • JMJD
      • Lysine-specific demethylase 4A
      • JmjC domain-containing histone demethylation protein 3A
      • Jumonji domain-containing protein 2A
      Background InformationJumonji domain-containing protein 2A (JMJD2A), or KDM4A, is a ubiquitously expressed demethylase enzyme, which belongs to the JMJD2 family. Like other members of this family, JMJD2A contains multiple domains: two Tudor domains, two PHD-type zinc fingers, one JmjC domain, and one JmjN domain. Little is known about the function of most of these domains; however, they are believed to be involved in transcriptional regulation. JMJD2A functions as a histone demethylase with specific activity against trimethylated Histone H3 Lysine residues Lys-9 and Lys-36. It has no effect on any other lysine residues, or on mono- and dimethylated Lys-9 and Lys-36. The demethylation of these residues induces the production of succinate and formaldehyde. Studies have shown that JMJD2A recruits NCOR1 and histone deacetylases, causing the respective transcriptional repression of E2Fand ASCL2 responsive promoters.
      References
      Product Information
      FormatPurified
      Control
      • U2OS nuclear extract
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-JMJD2A Antibody, clone 8B7.1 is validated for use in Western Blotting, IHC for the detection of JMJD2A.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:100 dilution from a representative lot detected JMJD2A in human colorectal adenocarcinoma cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human JMJD2A.
      Clone8B7.1
      HostRabbit
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KDM4A
      • JHDM3A
      • JMJD2
      • KIAA0677
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~120 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in U2OS nuclear extract.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected JMJD2A in 10 µg of U2OS nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE222 04053252698583

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies