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565834 sFRP-3, His•Tag®, Mouse, Recombinant

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
565834
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Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE

Products

Catalogue NumberPackaging Qty/Pack
565834-10UG Glass bottle 10 μg
Description
OverviewRecombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
Catalogue Number565834
Brand Family Calbiochem®
References
ReferencesHoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.
Product Information
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityProliferation Assay:
ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.

Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotxin: ≤1 EU/µg
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
565834-10UG 04055977191912

Documentation

sFRP-3, His•Tag®, Mouse, Recombinant SDS

Title

Safety Data Sheet (SDS) 

sFRP-3, His•Tag®, Mouse, Recombinant Certificates of Analysis

TitleLot Number
565834

References

Reference overview
Hoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-Sepmtember-2011 RFH
DescriptionRecombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
FormLyophilized
FormulationLyophilized from 0.2 µm filtered PBS, BSA.
Purity≥95% by SDS-PAGE
ContaminantsEndotxin: ≤1 EU/µg
Biological activityProliferation Assay:
ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.

Functional ELISA:
50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
SolubilityReconstitute with sterile PBS contanining ≥1% human or bovine serum albumin. Please refer to the vial label for lot-specific reconstitution volume.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesHoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.