71364 Sigma-AldrichpRSF-2 Ek/LIC Vector Kit - Novagen
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Overview
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Description | |
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Overview | This product has been discontinued. The pRSF-2 Ek/LIC Vector is a LIC-compatible derivative of pRSF-1b. The plasmid contains a strong T7lac promoter and an amino-terminal His•Tag® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pCDF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors. |
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form. | |
Catalogue Number | 71364 |
Brand Family | Novagen® |
References | |
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References | 1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356. |
Product Information | ||
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• | 1 µg | Ek/LIC Vector |
• | 25 units | T4 DNA Polymerase, LIC-qualified |
• | 50 µl | 25 mM EDTA |
• | 40 µl | 25 mM dATP |
• | 8 µl | Ek/LIC β-gal Control Insert |
• | 100 µl | 100 mM DTT |
• | 50 µl | 10X T4 DNA Polymerase Buffer |
• | 1.5 ml | Nuclease-free Water |
• | 5 × 2 ml | SOC Medium |
• | 10 µl | Test Plasmid |
• | 0.2 ml | BL21(DE3)pLysS Competent Cells |
• | 0.2 ml | BL21(DE3) Competent Cells |
• | 22 × 50 µl | NovaBlue GigaSingles™ Competent Cells |
Quality Level | MQ100 |
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Safety Information according to GHS |
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Safety Information |
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Product Usage Statements |
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Storage and Shipping Information | |
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Ship Code | Dry Ice Only |
Toxicity | Multiple Toxicity Values, refer to MSDS |
Storage | ≤ -70°C |
Do not freeze | Ok to freeze |
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Supplemental Information |
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Specifications |
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Global Trade Item Number | |
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Catalogue Number | GTIN |
71364 | 0 |
Documentation
pRSF-2 Ek/LIC Vector Kit - Novagen Certificates of Analysis
Title | Lot Number |
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71364 |
References
Reference overview |
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1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356. |
User Protocols
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TB053 Academic and Non-profit Laboratory Assurance Letter |
TB163 Ek/LIC Cloning Kits |
TB401 pRSF and pCDF Vectors |
Vector Map
Title |
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TB395VM pRSF-2 Ek/LIC Vector Map |
Vector Sequence
Title |
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pRSF-2 Ek/LIC Vector Sequence |