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71733 pIEx/Bac™-5 3C/LIC Vector Kit

pIEx/Bac™-5 3C/LIC Vector Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





The pIEx/Bac™-5 3C/LIC vector carries an N-terminal GST•Tag™ coding sequence followed by a recognition site for the human rhinovirus (HRV) 3C protease. This protease is highly specific for cleavage of the sequence LEVLFQ_GP, and is active at low temperatures. The 3C/LIC cloning site is followed by an optional C-terminal thrombin recognition site and a Strep•Tag® II coding sequence. This 3C/LIC vector is ready for rapid, directional cloning of PCR-amplified DNA, without restriction enzyme digestion or ligation. The pIEx/Bac vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus.
Catalogue Number71733
Brand Family Novagen®
References
Product Information
•20 µg (40 µl) 0.5 µg/µl pIEx/Bac-5 3C/LIC DNA
•50 µlT4 DNA Polymerase Buffer
•5 µg (10 µl) 0.5 µg/µl pIEx/Bac 3C/LIC Positive Expression Control
•25 UT4 DNA Polymerase, LIC-qualified
•100 µl100 mM DTT
•40 µl25 mM dATP
•50 µl25 mM EDTA
•1.5 ml Nuclease-free Water
•1 pkgNovaBlue GigaSingles Competent Cells
•2 mlSOC Medium
•10 µlTest Plasmid
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 22-36/37/38

Harmful if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Harmful
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71733 0