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71243 pIEx™-3 DNA

pIEx™-3 DNA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: InsectDirect™ System- pIEx™-3 DNA

71243
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-3 contains the coding sequence for the signal peptide of adipokinetic hormone (AKH) to allow secretion of the expressed protein. pIEx-3 also contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. Unique sites are shown on the circle map. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-3 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

For sequencing or PCR of recombinants in pIEx-3, refer to the table below to choose the appropriate primers.
Vector5' Primer3' Primeralt 5' Primer
pIEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
pIEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
pIEx-3S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
pIEx-4IE1 Promoter PrimerAS S•Tag 18mer Primer
NA
pIEx-5IE1 Promoter PrimerAS S•Tag 18mer Primer
NA
pBiEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
T7 Promoter Primer
pBiEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
T7 Promoter Primer
pBiEx-3IE1 Promoter PrimerAS S•Tag 18mer PrimerT7 Promoter Primer
Catalogue Number71243
Brand Family Novagen®
SynonymsInsectDirect™ System- pIEx™-3 DNA
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71243 0