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69862 pET-26b(+) DNA - Novagen

69862
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69862-3 Plastic ampoule 10 μg
Description
OverviewThe pET-26b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB071).

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69862
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69862-3 04055977258837

Documentation

pET-26b(+) DNA - Novagen Certificates of Analysis

TitleLot Number
69862

Citations

Title
  • Wu. L., et al. (2009) Structural Basis for Proteolytic Specificity of the Human Apoptosis-Inducing Granzyme M J. Immunol. 183, 421.
  • Damien Bessière, et al. (2008) Structure-function analysis of the THAP zinc finger of THAP1, a large C2CH DNA-binding module linked to Rb/E2F pathways. Journal of Biological Chemistry 283, 4352-4363.
  • Nobutaka Funa, Takayoshi Awakawa and Sueharu Horinouchi. (2007) Pentaketide resorcylic acid synthesis by Type III polyketide synthase from Neurospora crassa. Journal of Biological Chemistry 282, 14476-14481.
  • Yongchao Li, Diana C. Irwin and David B. Wilson. (2007) Processivity, substrate binding, and mechanism of cellulose hydrolysis by Thermobifida fusca Cel9A. Applied and Enviornmental Microbiology 73, 3165-3172.
  • Jean-Michel Heraud, et al. (2006) Subunit recombinant vaccine protects against monkeypox. Journal of immunology 177, 2552-2564.
  • Stan J. J. Brouns, et al. (2005) Engineering a selectable marker for hyperthermophiles. Journal of Biological Chemistry 280, 11422-11431.
  • Nobutaka Funa, et al. (2005) A novel quinone-forming monooxygenase family involved in modification of aromatic polyketides. Journal of Biological Chemistry 280, 14514-14523.
  • Ryohei Ishii, et al. (2005) Crystal structure of the tRNA 3' processing endoribonuclease tRNase Z from Thermotoga maritima. Journal of Biological Chemistry 280, 14138-14144.
  • Hongmin Li, et al. (2005) Crystal structures of T cell receptor β chains related to rheumatoid arthritis. Protein Science 14, 3025-3038.
  • Taishi Tsubouchi, et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. Journal of Biological Chemistry 280, 18511-18516.
  • Florence Vincent, Gideon J. Davies and James A. Brannigan. (2005) Structure and kinetics of a monomeric glucosamine 6-phosphate deaminase: missing link of the NagB superfamily? Journal of Biological Chemistry 280, 19649-19655.
  • Yu-hang Chen, et al. (2004) Structural basis of the α1-β subunit interaction of voltage-gated Ca2+ channels. 429, 675-680.
  • Watchrra Teartasin, et al. (2004) Expression, purification, and preliminary X-ray diffraction analysis of a ketoreductase from a type II polyketide synthase. Acta Crystallographica D60, 1137-1138.
  • Shalom D. Goldberg, et al. (2003) Identification of residues critical for catalysis in a class C b-lactamase by combinatorial scanning mutagenesis. Protein Science 12, 1633-1645.
  • User Protocols

    Title
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual

    Vector Map

    Title
    TB071VM pET-26b(+) Vector Map