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533872 miR-122 Inhibitor, NSC5476 - CAS 5455-90-3 - Calbiochem

Selectively down-regulates both mature miR-122 and primary miR-122 intracellular levels and reduces HIV replication.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NSC-5476, MLS000736518, microRNA-122 Inhibitor, 6-[(4aR,8aS)-(Octahydroquinolin-1(2H)-ylsulfonyl)-1,2,3,4-tetrahydroquinoline, 6-[(4aR,8aS)-(Octahydro-1(2H)-quinolinylsulfonyl)-1,2,3,4-tetrahydroquinoline, pri-miR-122 Transcription Inhibitor, miR-122 Inhibitor

Primary Target: miR-122
533872
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
5455-90-3C₁₈HN₂O₂S

Products

Catalogue NumberPackaging Qty/Pack
5.33872.0001 Glass bottle 10 mg
Description
OverviewA cell-permeable trans-decahydroquinolinylsulfonamide compound that acts as a potent, selective, and reversible inhibitor of miR-122 (EC50 = 600 nM in Huh7 cells transfected with psiCHECK-miR122 reporter plasmid). Induces a 1,250% increase in the relative luciferase signal that is indicative of strong miRNA-122 inhibition. Displays very little activity towards miR-21. Shown to down regulate the levels of mature miR-122 (by 72%) and the primary miR-122 (down to 3%). Shown to reduce hepatitis C virus (HCV) replication in Huh7 cells (47% reduction at 10 µM).

Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
Catalogue Number533872
Brand Family Calbiochem®
SynonymsNSC-5476, MLS000736518, microRNA-122 Inhibitor, 6-[(4aR,8aS)-(Octahydroquinolin-1(2H)-ylsulfonyl)-1,2,3,4-tetrahydroquinoline, 6-[(4aR,8aS)-(Octahydro-1(2H)-quinolinylsulfonyl)-1,2,3,4-tetrahydroquinoline, pri-miR-122 Transcription Inhibitor, miR-122 Inhibitor
DescriptionmiR-122 Inhibitor, NSC5476
References
ReferencesYoung, D.D., et al. 2010. J. Am. Chem. Soc. 132, 7976.
Connelly, C.M., et al. 2012. J. Biomol. Screen. 17, 822.
Product Information
CAS number5455-90-3
FormWhite solid
Hill FormulaC₁₈HN₂O₂S
Chemical formulaC₁₈HN₂O₂S
ReversibleY
Quality LevelMQ100
Applications
Biological Information
Primary TargetmiR-122
Purity≥98% by HPLC
Physicochemical Information
Cell permeableY
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
5.33872.0001 04055977286809

Documentation

miR-122 Inhibitor, NSC5476 - CAS 5455-90-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

References

Reference overview
Young, D.D., et al. 2010. J. Am. Chem. Soc. 132, 7976.
Connelly, C.M., et al. 2012. J. Biomol. Screen. 17, 822.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-September-2016 JSW
SynonymsNSC-5476, MLS000736518, microRNA-122 Inhibitor, 6-[(4aR,8aS)-(Octahydroquinolin-1(2H)-ylsulfonyl)-1,2,3,4-tetrahydroquinoline, 6-[(4aR,8aS)-(Octahydro-1(2H)-quinolinylsulfonyl)-1,2,3,4-tetrahydroquinoline, pri-miR-122 Transcription Inhibitor, miR-122 Inhibitor
DescriptionA cell-permeable trans-decahydroquinolinylsulfonamide compound that downregulates the liver-specific microRNA miR-122 level and pri-miR-122 production in Huh7 cells (by 72% & 93%, respectively; 10 µM for 48 h) without interaction with pre-miR-123 or affecting cellular miR-21 level. Selectively prevents miR-122-, but not miR-21-, mediated reporter transcription blockage (10 µM for 48 h) and suppresses HCV replication (by 53%; 10 µM inhibitor added 4 h post H77c/H77c/QR/KR/FV/SI RNA transfection for 48 h) in Huh7 cultures without affecting Huh7 proliferation.
FormWhite solid
Intert gas (Yes/No) Packaged under inert gas
CAS number5455-90-3
Chemical formulaC₁₈HN₂O₂S
Purity≥98% by HPLC
SolubilityDMSO (50 mg/ml)
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesYoung, D.D., et al. 2010. J. Am. Chem. Soc. 132, 7976.
Connelly, C.M., et al. 2012. J. Biomol. Screen. 17, 822.