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475987 mTOR, Human, Recombinant, S. frugiperda

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475987
  
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Overview

Replacement Information
Description
OverviewRecombinant, human mTOR consisting of amino acids 1360-2549 fused at the N-terminus to DYKDDDDK sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. mTOR is a ubiquitously expressed, serine/threonine protein kinase regulated by growth factor signaling, amino acid availability, and energy levels. mTOR activity is inhibited by rapamycin as well as broad spectrum PI 3-kinase inhibitors. The catalytically active enzyme is useful for inhibitor screening. Active in kinase assay using the K-LISA™ mTOR (Recombinant) Activity Kit, Cat. No. CBA104.

This product has been discontinued.
Catalogue Number475987
Brand Family Calbiochem®
Application Data
Increasing amounts of mTOR, Human, Recombinant, S. frugiperda were assayed for mTOR activity using the K-LISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.

The activity of mTOR, Human, Recombinant, S. frugiperda was assaying in the presence of increasing amounts of Wortmannin (Cat. No. 681675) using the KLISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.

The activity of mTOR, Human, Recombinant, S. frugiperda was assayed in the presence of increasing concentrations of Rapamycin (Cat. No. 553210) and in the presence (+) or absence (-) of FKBP12, GST-Fusion, Human, Recombinant, E. coli (325902) using the KLISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.
References
ReferencesSarbassov, D.D., et al. 2005. Science 307, 1098.
Chiang, G.G. and Abraham, R.T. 2004. Methods Mol. Biol. 281, 125.
Sarbassov, D.D., et al. 2004. Curr. Biol. 14,1296.
Kim, D.H., et al. 2002. Cell 110,163.
Brunn, G.J., et al. 1996. EMBO J. 15, 5256.
Brown, E.J., et al. 1994. Nature 369,756.
Sabatini, D.M., et al. 1994. Cell 78, 35.
Heitman, J., et al. 1991. Science 253, 905.
Product Information
FormLiquid
FormulationIn 50 mM NaCl, 50 mM β-glycerophosphate, 10 mM HEPES, 500 µM TCEP, 10% glycerol, 0.03% Brij-®35 detergent, 200 µg/ml DYKDDDDK Peptide, pH 7.4.
Applications
Biological Information
Purity≥50% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
475987 0

Documentation

mTOR, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
475987

References

Reference overview
Sarbassov, D.D., et al. 2005. Science 307, 1098.
Chiang, G.G. and Abraham, R.T. 2004. Methods Mol. Biol. 281, 125.
Sarbassov, D.D., et al. 2004. Curr. Biol. 14,1296.
Kim, D.H., et al. 2002. Cell 110,163.
Brunn, G.J., et al. 1996. EMBO J. 15, 5256.
Brown, E.J., et al. 1994. Nature 369,756.
Sabatini, D.M., et al. 1994. Cell 78, 35.
Heitman, J., et al. 1991. Science 253, 905.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-April-2009 JSW
Application Data
Increasing amounts of mTOR, Human, Recombinant, S. frugiperda were assayed for mTOR activity using the K-LISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.

The activity of mTOR, Human, Recombinant, S. frugiperda was assaying in the presence of increasing amounts of Wortmannin (Cat. No. 681675) using the KLISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.

The activity of mTOR, Human, Recombinant, S. frugiperda was assayed in the presence of increasing concentrations of Rapamycin (Cat. No. 553210) and in the presence (+) or absence (-) of FKBP12, GST-Fusion, Human, Recombinant, E. coli (325902) using the KLISA™ mTOR (Recombinant) Activity Kit (Cat. No. CBA104), as indicated in the Detailed Protocol of the kit.
DescriptionRecombinant, human mTOR consisting of amino acids 1360-2549 fused at the N-terminus to DYKDDDDK sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. mTOR is a ubiquitously expressed, serine/threonine protein kinase regulated by growth factor signaling, amino acid availability, and energy levels. mTOR activity is inhibited by rapamycin as well as broad spectrum PI 3-kinase inhibitors. The catalytically active enzyme is useful for inhibitor screening. Active in kinase assay using the K-LISA™ mTOR (Recombinant) Activity Kit, Cat. No. CBA104.
FormLiquid
FormulationIn 50 mM NaCl, 50 mM β-glycerophosphate, 10 mM HEPES, 500 µM TCEP, 10% glycerol, 0.03% Brij-®35 detergent, 200 µg/ml DYKDDDDK Peptide, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥50% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesSarbassov, D.D., et al. 2005. Science 307, 1098.
Chiang, G.G. and Abraham, R.T. 2004. Methods Mol. Biol. 281, 125.
Sarbassov, D.D., et al. 2004. Curr. Biol. 14,1296.
Kim, D.H., et al. 2002. Cell 110,163.
Brunn, G.J., et al. 1996. EMBO J. 15, 5256.
Brown, E.J., et al. 1994. Nature 369,756.
Sabatini, D.M., et al. 1994. Cell 78, 35.
Heitman, J., et al. 1991. Science 253, 905.