Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

71719 iFOLD® Protein Refolding System 2

iFOLD® Protein Refolding System 2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71719
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





The iFOLD® Protein Refolding System 2 is a detergent-free kit to screen for optimal refolding conditions for proteins found in inclusion bodies. The kit provides a systematic evaluation of 95 conditions representing different buffering systems, pH values, and combinations of salt, redox agent, and refolding additives. The system consists of inclusion body purification reagents and a pre-dispensed 96-well plate-based matrix of refolding buffers. Following cell lysis, membrane components and contaminating proteins trapped within inclusion body pellets are removed by a series of washes with a non-detergent sulfobetaine (NDSB). The purified inclusion bodies are reduced and denatured by addition of Tris(2-carboxyethyl) phosphine (TCEP) and guanidine hydrochloride and refolded by rapid dilution into the iFOLD System 2 detergent-free 96-well buffer matrix. Each kit is sufficient for screening up to 96 refolding conditions (95 experimental and 1 control well) for a single protein.
Catalogue Number71719
Brand Family Novagen®
References
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Multiple Storage Conditions
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71719 0

Documentation

iFOLD® Protein Refolding System 2 Certificates of Analysis

TitleLot Number
71719

User Protocols

Title
TB467