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71252 Veggie BL21(DE3) Singles™ Competent Cells - Novagen

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71252
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
71252-3 Plastic ampoule 11 rxn
71252-4 Glass bottle 22 rxn
Description
OverviewBL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3)


This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number71252
Brand Family Novagen®
Features and benefits
  • Manufactured free of animal-derived media and components
  • Protease-deficient hosts (BL21) for protein expression
  • Easy-to-use Singles format
  • Cells made competent by a chemical method
References
Product Information
•11 rxn or 22 rxnVeggie BL21(DE3) Competent Cells
•2 × 2 ml or 4 × 2 mlSOC Veggie™ Medium
•10 µlTest Plasmid
DeclarationVeggie and Singles are trademarks of EMD Biosciences, Inc., Madison, WI USA
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71252-3 04055977270839
71252-4 04055977270846