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23-047 UbcH7 Protein, active, 10 µg

Active, full length, recombinant human UbcH7. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.

UbcH7 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
23-047
10 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpeciesCascade Component
Enzyme AssaysHuman E2
Description
Catalogue Number23-047
Brand Family Upstate
Trade Name
  • Upstate
DescriptionUbcH7 Protein, active, 10 µg
Alternate Names
  • Ubiquitin-conjugating enzyme E2 L3
  • L-UBC
  • Ubiquitin carrier protein L3
  • Ubiquitin-conjugating enzyme E2-F1
  • Ubiquitin-protein ligase L3
  • UBE2L3
  • UBCE7
Background InformationUbiquitin-conjugating (E2) enzymes are characterized by the presence of a highly conserved ubiquitin-conjugating domain, which accommodates ATP-activated ubiquitin (Ub) via a covalently linked thioester on its active-site residue. E2 enzymes act through selective protein-protein interactions with the ubiquitin-activating E1 enzyme and ubiquitin ligase E3 enzymes and are able to differentiate effects on downstream substrates, either with a single Ub molecule or a Ub chain. While E3s are involved in substrate selection, E2s are the main determinants for selection of the lysine to construct Ub chains, which thereby directly control the cellular fate of the substrate.

UbcH7 shows broad specificity for HECT-type E3s but does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine. Despite lacking this lysine reactivity, UbcH7 exhibits activity with the RBR family E3 enzymes, such as Parkin and ARIH1.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, full length, recombinant human UbcH7. N-terminal HA, 6His-tagged. For use in Enzyme Assays. Functions as an E2 enzyme in ubiquitination assays.
Key Applications
  • Enzyme Assays
Biological Information
Cascade ComponentE2
Entrez Gene Number
Purification MethodPurified using immobilized metal affinity chromatography.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
23-047 04053252596865

Related Products & Applications

Alternative Packsize

Catalogue Number Description
23-047M UbcH7 Protein, active, 250 µg

Product Families

Ubiquitin Proteasome System: Enzymes and Substrates

Millipore has removed the complexity of Ubiquitination by providing functionally validated enzymes and substrates, making the pathway readily accessible for research and drug discovery.
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Categories

Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitin E2 Conjugating Enzymes
Life Science Research > Proteins and Enzymes > Ubiquitin E2 Conjugating Enzymes