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654161 Tubulin-α-1, His•Tag® Fusion, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Tubulin-α-1, Human, Recombinant

654161
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.





Full-length, recombinant, human tubulin-α-1 (TBA1, TUBA1, α-tubulin 1) fused to His•Tag® and S•Tag™ sequences at the N-terminus and expressed in E. coli. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Tubulin-α-1 is a major constituent of microtubules. It binds one GTP at a nonexchangeable site (aa 142-148) on the alpha-chain. IFN-α-induced phosphorylation at Tyr271 has been demonstrated in Jurkat cells. Tyrosine phosphorylation is expected at Tyr172, Tyr210, Tyr224, and Tyr282 by EGFR and at Tyr440 by InsR.
Catalogue Number654161
Brand Family Calbiochem®
SynonymsTubulin-α-1, Human, Recombinant
Application Data
References
ReferencesLennon, G., et al. 1996. Genomics 33, 151.
Cox, M.E. and Maness, P.F. 1993. J. Mol. Neurosci. 4, 63.
Matten, W.T., et al. 1990. J. Cell. Biol. 111, 1959.
Product Information
FormLiquid
FormulationIn PBS, 6 M Urea, 0.2% Protease Inhibitor Cocktail Set VII (Cat. No. 539138).
Applications
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
654161 0

Documentation

Tubulin-α-1, His•Tag® Fusion, E. coli Certificates of Analysis

TitleLot Number
654161

References

Reference overview
Lennon, G., et al. 1996. Genomics 33, 151.
Cox, M.E. and Maness, P.F. 1993. J. Mol. Neurosci. 4, 63.
Matten, W.T., et al. 1990. J. Cell. Biol. 111, 1959.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-September-2008 RFH
SynonymsTubulin-α-1, Human, Recombinant
Application Data
DescriptionFull-length, recombinant, human tubulin-α-1 (TBA1, TUBA1, α-tubulin 1) fused to His•Tag® and S•Tag™ sequences at the N-terminus and expressed in E. coli. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Tubulin-α-1 is a major constituent of microtubules. It binds one GTP at a nonexchangeable site (aa 142-148) on the alpha-chain. IFN-α-induced phosphorylation at Tyr271 has been demonstrated in Jurkat cells. Tyrosine phosphorylation is expected at Tyr172, Tyr210, Tyr224, and Tyr282 by EGFR and at Tyr440 by InsR.
FormLiquid
FormulationIn PBS, 6 M Urea, 0.2% Protease Inhibitor Cocktail Set VII (Cat. No. 539138).
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Irritant
ReferencesLennon, G., et al. 1996. Genomics 33, 151.
Cox, M.E. and Maness, P.F. 1993. J. Mol. Neurosci. 4, 63.
Matten, W.T., et al. 1990. J. Cell. Biol. 111, 1959.