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6502 Trypsin, Bovine Pancreas

Trypsin, Bovine Pancreas MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
6502
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
6502-2.5MU Glass bottle 2.5 mu
6502-25MU Plastic ampoule 25 mu
Description
OverviewNative trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units.
Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units.
Catalogue Number6502
Brand Family Calbiochem®
References
ReferencesBergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799.
Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.
Product Information
CAS number9002-07-7
Activity≥2,500 USP units/mg dry weight
Unit of DefinitionOne USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
EC number3.4.21.4
FormSolid
FormulationSalt-free
Hygroscopic Hygroscopic
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
ContaminantsChymotrypsin: ≤50 NF units/mg dry weight
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSYN5075000
Safety Information
R PhraseR: 36/37/38-42

Irritating to eyes, respiratory system and skin.
May cause sensitization by inhalation.
S PhraseS: 22-24-26-36/37

Do not breathe dust.
Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
6502-2.5MU 04055977185140
6502-25MU 04055977185157

Documentation

Trypsin, Bovine Pancreas SDS

Title

Safety Data Sheet (SDS) 

Trypsin, Bovine Pancreas Certificates of Analysis

TitleLot Number
6502

References

Reference overview
Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799.
Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.

Citations

Title
  • Elizabeth A. Lipscomb, et al. (2005) The α6β4 integrin maintains the survival of human breast carcinoma cells in vivo. Cancer Research 65, 10970-10976.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision19-May-2008 RFH
    DescriptionNative trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Has an optimal pH of 8.5-8.8.
    FormSolid
    FormulationSalt-free
    CAS number9002-07-7
    RTECSYN5075000
    EC number3.4.21.4
    ContaminantsChymotrypsin: ≤50 NF units/mg dry weight
    Activity≥2,500 USP units/mg dry weight
    Unit definitionOne USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
    SolubilityHCl (1 mM), H₂O, or Neutral Buffers
    Storage -20°C
    Hygroscopic
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.
    Toxicity Irritant
    Merck USA index14, 9795
    ReferencesBergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
    Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799.
    Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.
    Citation
  • Elizabeth A. Lipscomb, et al. (2005) The α6β4 integrin maintains the survival of human breast carcinoma cells in vivo. Cancer Research 65, 10970-10976.
    		•