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14-766M Tie2 (Y1108F) Protein, active, 250 µg

Active, N-terminal 6His-tagged recombinant human Tie2 residues 771-end. For use in Kinase Assays.

Tie2 (Y1108F) Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-766M
250 µg  Also available in 10 μg size (cat#14-766) and in bulk (cat# 14-766-K).
Purchase on Sigma-Aldrich

Overview

Description
Catalogue Number14-766M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionTie2 (Y1108F) Protein, active, 250 µg
OverviewN-terminal 6His-tagged recombinant human Tie2 residues 771-end.
Background InformationTyrosine 1108 has been identified as an autophosphorylation site in human Tie2. The Y1106F substitution in murine Tie2 (analogous to Y1108F in human Tie2) has been shown to block cell migration by disrupting binding of Tie2 to the docking protein Dok-R (Jones N., et al., Mol Cell Biol.(2003);23:2658-2668 and Murray B.W., et al., Biochemistry.(2001);40:10243-10253).
Product Information
Presentation50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Quality LevelMQ300
Applications
ApplicationActive, N-terminal 6His-tagged recombinant human Tie2 residues 771-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed by baculovirus in Sf21 insect cell.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • TEK
  • TIE2
Protein TargetTie2
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyTK
UniProt Number
Molecular Weight42kDa
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Poly (Glu4-Tyr) (4:1)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-766) and in bulk (cat# 14-766-K).
Global Trade Item Number
Catalogue Number GTIN
14-766M 04053252411847