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GF037 Thrombopoietin

TPO (Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation & maturation of megakaryocytes & promotes increased circulating levels of platelets in vivo.

Thrombopoietin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P40225 Entrez Gene Number: NM_199228.1, NM_199356.1, NM_000460.2
GF037
10 µg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSpeciesSourcePurity
CULTHuman E.coliGreater than 98% by SDS-PAGE and HPLC analyses. . Endotoxin level is less than 0.1 ng per mg (1 EU/μg) of human TPO.
Description
Catalogue NumberGF037
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionThrombopoietin
OverviewTPO (Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo. Chemicon's Human TPO is a soluble 18.5 kDa protein containing 174 amino acid residues which comprises the receptor binding domain of the Mpl-ligand protein.
Alternate Names
  • TPO
References
Product Information
PresentationLyophilized protein from 5mM Tris, pH 7.6, with no additives.
Quality LevelMQ100
Applications
ApplicationTPO (Thrombopoietin, Mpl-ligand, MGDF) stimulates the proliferation & maturation of megakaryocytes & promotes increased circulating levels of platelets in vivo.
Key Applications
  • Cell Culture
Biological Information
PurityGreater than 98% by SDS-PAGE and HPLC analyses. . Endotoxin level is less than 0.1 ng per mg (1 EU/μg) of human TPO.
SourceE.coli
Specific ActivityThe ED50 was determined by the dose-dependent stimulation of the proliferation of M07e cells is <=1.0 ng/mL, corresponding to a specific activity of >=1x10(e6) units/mg.
Entrez Gene Number
Entrez Gene SummaryMegakaryocytopoiesis is the cellular development process that leads to platelet production. The protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene.
Gene Symbol
  • THPO
  • TPO
  • MGC163194
  • MPLLG
  • thrombopoietin
  • MGDF
  • ML
  • MKCSF
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P40225 # Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
SIZE: 353 amino acids; 37823 Da
SUBCELLULAR LOCATION: Secreted.
DOMAIN: SwissProt: P40225 Two-domain structure with an erythropoietin-like N- terminal and a Ser/Pro/Thr-rich C-terminal.
DISEASE: SwissProt: P40225 # Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
SIMILARITY: Belongs to the EPO/TPO family.
Stem Cell Type
  • Hematopoietic Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe lyophilized powder, though stable at room temperature, is best stored at -20°C. Reconstitute in 5 mM Tris, pH 8.0 to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other aqueous buffers and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted TPO should be stored in working aliquots at -20°C.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF037 08436037125003

Documentation

Thrombopoietin SDS

Title

Safety Data Sheet (SDS) 

Thrombopoietin Certificates of Analysis

TitleLot Number
RECOMBINANT HUMAN THROMBOPOIETIN 2462796
RECOMBINANT HUMAN THROMBOPOIETIN 2991003
RECOMBINANT HUMAN THROMBOPOIETIN - 2121736 2121736
RECOMBINANT HUMAN THROMBOPOIETIN - 2012361 2012361
RECOMBINANT HUMAN THROMBOPOIETIN - 2025949 2025949
RECOMBINANT HUMAN THROMBOPOIETIN - 2048774 2048774
RECOMBINANT HUMAN THROMBOPOIETIN - 2089385 2089385
RECOMBINANT HUMAN THROMBOPOIETIN - 2196002 2196002
RECOMBINANT HUMAN THROMBOPOIETIN - 2271152 2271152
RECOMBINANT HUMAN THROMBOPOIETIN - 2352339 2352339
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Data Sheet

Title
RECOMBINANT HUMAN THROMBOPOIETIN

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Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors