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14-628M TBK1 Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, full length, human TBK1, for use in Kinase Assays.

TBK1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-628M
250 µg  Also available in 10 μg size (cat#14-628) and in bulk (cat# 14-628-K).
Purchase on Sigma-Aldrich

Overview

Description
Catalogue Number14-628M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionTBK1 Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant, full length, human TBK1
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal His6-tagged, recombinant, full length, human TBK1, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK.
Gene Symbol
  • TBK1
  • T2K
  • NAK
  • FLJ11330
Protein TargetTBK1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyMisc
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q9UHD2 # Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
SIZE: 729 amino acids; 83642 Da
SUBUNIT: Interacts with TIRAP, TANK and TRAF2. Part of a ternary complex consisting of TANK, TRAF2 and TBK1. Interacts with AZI2. Interacts with SIKE. Interacts with TICAM1/TRIF, IRF3 and DDX58/RIG-I, interactions are disrupted by the interaction between TBK1 and SIKE. Interacts with HCV NS3, a hepatitis C virus protein and with BDV P protein, a Borna disease virus protein.
SUBCELLULAR LOCATION: Cytoplasm.TISSUE SPECIFICITY: Ubiquitous with higher expression in testis.
SIMILARITY: SwissProt: Q9UHD2 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight85.7kDa
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of CK1tide (12-529)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-628) and in bulk (cat# 14-628-K).
Global Trade Item Number
Catalogue Number GTIN
14-628M 04053252411472