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574601 Superoxide Dismutase Assay Kit II

Superoxide Dismutase Assay Kit II MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
574601
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Overview

Products

Catalogue NumberPackaging Qty/Pack
574601-1KIT Glass bottle 1 kit
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Description
OverviewA sensitive spectrophotometric assay (450 nm) for the measurement of all three types (Cu/Zn, Mn, and Fe) of SOD.
Catalogue Number574601
Brand Family Calbiochem®
Materials Required but Not Delivered• Plate reader capable of reading absorbance at 450 nm.
• Adjustable pipettors and a repeat pipettor.
• A source of pure water. Glass distilled water or HPLC-grade water is acceptable.
References
ReferencesMaier, C.M. and Chan, P.H 2002. The Neuroscientist 8, 323.
Mattiazz, M., et al. 2002. J. Biol. Chem. 277, 29626.
Beckman, J.S. and Koppenol, W.H. 1996. Am. J. Physiol. 271, C1424.
Liu, D. 1996. J. Mol. Neuro. 7, 159.
MacMillan-Crow, L.A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11853.
Sun, E., et al. 1995. Biol. Trace Elem. Res. 48, 231.
Sandstrom, J., et al. 1994. J. Biol. Chem. 269, 19163.
Marklund, S. 1980. Acta Physiol. Scand. Suppl. 492, 19.
Malstrom, B. 1975. in The Enzymes. Boyer, P., editor. XIIB, Academic Press, New York, 533.
Product Information
Unit of DefinitionThe amount of enzyme needed to exhibit 50% dismutation of the superoxide radical.
Detection methodColorimetric
Form96 Tests
Format96-well plate
Kit containsAssay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol.
Positive controlBovine erythrocyte SOD (Cu/Zn)
Quality LevelMQ100
Biological Information
Assay range0.025-0.25 units per ml SOD
Assay time1.5 h
Sample TypePlasma, serum, erythrocyte lysates, and other lysates, tissue homogenates, and cell lysates
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Storage ConditionsUpon arrival store entire contents of the kit at -20°C.
Do not freeze Ok to freeze
Supplemental Information
Kit containsAssay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol.
Global Trade Item Number
Catalogue Number GTIN
574601-1KIT 07790788051822