Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

12-140 Src Substrate Peptide

Src Substrate Peptide primarily used in Kinase Assays.

Src Substrate Peptide MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
12-140
1 mg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number12-140
Brand Family Upstate
Trade Name
  • Upstate
DescriptionSrc Substrate Peptide
OverviewSynthetic peptide (KVEKIGEGTYGVVYK) corresponding to amino acids 6-20 of p34cdc2.
References
Product Information
PresentationLyophilized powder
Quality LevelMQ100
Applications
ApplicationSrc Substrate Peptide primarily used in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Entrez Gene Number
Entrez Gene SummaryThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol
  • SRC
  • ASV
  • c-src
  • p60-Src
  • SRC1
  • c-SRC
  • pp60c-src
Protein TargetSrc
Target Sub-FamilyTK
UniProt Number
UniProt SummarySIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight1670 Daltons (1.67 kDa)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated in a protein kinase assay as a substrate for Src (p60c-src), (14-117) or Lck (p56lck), active (14-379).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 2 years at -20°C
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
12-140 04053252337550

Documentation

Src Substrate Peptide SDS

Title

Safety Data Sheet (SDS) 

Src Substrate Peptide Certificates of Analysis

TitleLot Number
16-184 - 2298361 2298361
Src Substrate Peptide 3291283
Src Substrate Peptide - 2148509 2148509
Src Substrate Peptide - 2439213 2439213
Src Substrate Peptide - 2439655 2439655
Src Substrate Peptide - 16332 16332
Src Substrate Peptide - 17808 17808
Src Substrate Peptide - 18651 18651
Src Substrate Peptide - 1984870 1984870
Src Substrate Peptide - 20579 20579
Show more

References

Reference overviewApplicationPub Med ID
Contraction regulation of Akt in rat skeletal muscle
Sakamoto, K., et al
J Biol Chem, 277:11910-7 (2002)  2002

Immunoblotting (Western)11809761 11809761
Eicosanoid activation of extracellular signal-regulated kinase1/2 in human epidermoid carcinoma cells
Szekeres, C. K., et al
J Biol Chem, 275:38831-41 (2000)  2000

Immunoblotting (Western)10952974 10952974
RACK1, a receptor for activated C kinase and a homolog of the beta subunit of G proteins, inhibits activity of src tyrosine kinases and growth of NIH 3T3 cells
Chang, B. Y., et al
Mol Cell Biol, 18:3245-56 (1998)  1998

Kinase Assay9584165 9584165

Related Products & Applications

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
Learn More >>

Categories

Life Science Research > Proteins and Enzymes > Substrate & Control Proteins > Kinase & Phosphatase Substrates
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Kinase & Phosphatase Substrates