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03-0193-00 SMC™ Human SARS-CoV-2 RBD IgG Kit

03-0193-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Single Molecule Counting (SMC®)
Description
Catalogue Number03-0193-00
Trade Name
  • SMC®
DescriptionSMC™ Human SARS-CoV-2 RBD IgG Kit
OverviewThe SMC™ SARS-CoV-2 RBD IgG Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationImmunoassay-based serology techniques play key roles in many SARS-CoV-2 research programs due to their ability to identify and characterize sample of infected individuals long after initial exposure. However, many traditional assay platforms, due to limitations in sensitivity and resolution, may not be truly optimal for use in situations where low-level characterization and stratification of SARS-CoV-2 antibody responses are essential, such as population studies, temporal infection timecourse experiments, and evaluating vaccine efficacy.
References
Product Information
Components
  • Assay Buffer
  • SARS-CoV-2 RBD IgG Beads
  • Standard Diluent
  • SARS-CoV-2 RBD IgG Detection Antibody
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • The assay variation of the SMC™ Human SARS-CoV-2 RBD IgG Immunoassay kit were studied using twenty-one plasma samples, with roughly half positive and half negative, run in triplicate by 2 different operators on 2 different days.
  • Mean intra-assay variation for all samples was < 15%.
  • Mean inter-assay variation for all samples was < 21%.
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ SARS-CoV-2 RBD IgG Kit unparalleled performance in the qualitative determination of SARS-CoV-2 RBD IgG in Human EDTA Plasma and Serum.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has the following features including:
• For use with the state of the art SMCxPRO™ Ultrasensitive Immunoassay System
• Qualitative assay; for research use only
• Detects SARS-CoV-2 RBD specific IgG antibody within 3 days of PCR positive result
• Requires as little as 2μL of human serum and plasma sample (1:50 recommended dilution)
• Fast assay protocol (one hour capture, 30 minute detection steps)
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-0193-00 04061841868415