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03-0017-05 SMC™ Human Interleukin 17A (IL-17A)

The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-17A Immunoassay Kit unparalleled performance in the quantification of IL-17A in human EDTA plasma and serum.

03-0017-05
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number03-0017-05
Trade Name
  • SMC®
DescriptionSMC™ Human Interleukin 17A (IL-17A)
OverviewThe SMC™ IL-17A Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin-17A (IL-17A) is the founding member of a group of cytokines called the IL-17 family. There are several members of the IL-17 family, from IL-17A to IL-17F (IL-17E is also called IL-45). IL-17A is involved in mediating proinflammatory responses by inducing the production of many other cytokines, chemokines, and prostaglandins from many cell types. The increased expression of chemokines attracts other cells, including neutrophils. IL-17A has many physiological functions, such as airway remodeling. As a result of these roles, the IL-17 family has been linked to many immune/autoimmune related diseases, including rheumatoid arthritis (RA), asthma, allergic responses, lupus, allograft rejection and anti-tumor immunity.
References
Product Information
Components
  • IL-17A Coated Beads
  • Standard Diluent
  • IL-17A Detection Antibody
  • Assay Buffer
  • IL-17A Standard
  • 10X Wash Buffer
  • Elution Buffer B
  • Buffer C
  • SMC™ Human Interleukin 17A (IL-17A) Immunoassay Kit Instructions
Precision, %
  • Low-end CV% Range: 1-13%
  • Low-end CV% Average: 6%
Quality LevelMQ300
Applications
ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-17A Immunoassay Kit unparalleled performance in the quantification of IL-17A in human EDTA plasma and serum.
Key Applications
  • Single Molecule Counting (SMC®)
Biological Information
Species Reactivity
  • Human
Analytes Available
  • IL-17A
Accuracy
  • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
Physicochemical Information
Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-0017-05 04055977317800

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