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03-0213-00 SMC® Human IFNλ1 High Sensitivity Immunoassay Kit

For ultrasensitive detection of IFNλ1 (IL-29) in human serum and plasma.

03-0213-00
96 well assay  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number03-0213-00
Trade Name
  • SMC®
DescriptionSMC® Human IFNλ1 High Sensitivity Immunoassay Kit
OverviewThe SMC® Human IFNλ1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMCxPRO® immunoassay systems detect analytes using SMC® technology.
Background InformationInterferon lambda 1 (IFN-λ1), also know as IL-29, is a type of cytokine belonging to the interferon lambda family, which also includes IL-28A, IL-28B, and IL-29. These cytokines are recognized for their role in the immune response against viral infections and are structurally similar to members of the IL-10 family. IL-29 is primarily produced by dendritic cells and is involved in the modulation of antiviral activity, acting similarly to type I interferons, though it interacts with a distinct receptor complex. This cytokine is crucial for its antiviral properties, immune regulation, and potential therapeutic implications in managing viral infections and certain autoimmune disorders. The specific signaling through its receptor can activate various pathways leading to the induction of antiviral state and regulation of immune responses.
References
Product Information
Components
  • Assay Buffer;IFNL1 Coated Beads;Standard Diluent;IFNL1 Detection Antibody;IFNL1 Standard;10X Wash Buffer;Buffer D;Elution Buffer B
Precision, %
  • Intra-/Inter-assay %CV: 5% and 9%, respectively
    (five normal plasma samples run in triplicate by 3 different operators on 3 different days).
Quality LevelMQ200
Applications
ApplicationFor ultrasensitive detection of IFNλ1 (IL-29) in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThe SMC® Human IFN-λ1 High Sensitivity Immunoassay uses a quantitative fluorescent sandwich immunoassay technique to measure IFN-λ1 in Human serum or plasma samples. A capture antibody specific for Human IFN-λ1 has been pre-coated onto paramagnetic microparticles (beads). The user pipettes beads, standards, and samples into uncoated microplate wells. During incubation, the IFN-λ1 present in the sample binds to the capture antibody on the coated beads. Unbound molecules are washed away during the subsequent wash steps. Fluor-labeled detection antibody is added to each well and incubated. This detection antibody recognizes and binds to IFN-λ1 that has been captured onto the beads, thus completing the sandwich. Elution buffer is added to dissociate the protein sandwich, releasing the fluor-labeled antibodies. The eluted antibodies are transferred to a SMC® 384-well Read Plate. The plate is loaded into the SMCxPRO® System where the labeled molecules are detected and counted. The number of fluor-labeled detection antibodies counted is directly proportional to the amount of IFN-λ1 present in the sample. The amount of IFN-λ1 in unknown samples is interpolated from a standard curve.
Biological Information
Cross ReactivityThis assay was tested for cross reactivity with IFN-ε, IFN-α2, IFN-γ, IFN-λ3, IFN-β, IFN-ω, and IFN-λ2 and no cross-reactivity was observed.
Accuracy
  • Spike recovery ranged between 61%-106% with a mean of 85% for serum and between 71% -120% with a mean of 95% for plasma.
Physicochemical Information
Sensitivity
  • Sensitivity (LLOQ): 0.26 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
03-0213-00 04065271124110

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