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524743 SIRT1, GST-Fusion, Human, Recombinant, E. Coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Silent mating type information regulation 2 homolog, Sirtuin1, GST-Fusion, Human, Recombinant, E. coli

524743
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Recombinant, human SIRT1 histone deacetylase consisting of amino acids 193-741 fused at the N-terminus to GST and expressed in E. coli. SIRT1 preferentially deacetylates histones H4 at K16 and H3 at K9 and interacts with and deacetylates histone H1 at K26. More than a dozen non-histone proteins have been found to serve as substrates for SIRT1. It plays a role in several biological processes including stress resistance, metabolism, differentiation, and aging. The active deacetylase is useful in the study of SIRT1 regulation and for inhibitor screening.
Catalogue Number524743
Brand Family Calbiochem®
SynonymsSilent mating type information regulation 2 homolog, Sirtuin1, GST-Fusion, Human, Recombinant, E. coli
References
ReferencesKolthur-Seetharam, U., et al. 2006. Cell Cycle 5, 873.
Solomon, J.M., et al. 2006. Mol. Cell. Biol. 26, 28.
Product Information
Unit of DefinitionOne unit is defined as the amount of enyme required to deacetylate 1 pmol fluorescent peptide substrate [Arg-His-Lys-Lys(Ac)], per min at 30°C.
FormLiquid
FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 20% glycerol, pH 8.0.
Quality LevelMQ100
Applications
Biological Information
Purity≥80% by SDS-PAGE
Specific Activity≥8 units/µg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
524743 0

Documentation

SIRT1, GST-Fusion, Human, Recombinant, E. Coli SDS

Title

Safety Data Sheet (SDS) 

SIRT1, GST-Fusion, Human, Recombinant, E. Coli Certificates of Analysis

TitleLot Number
524743

References

Reference overview
Kolthur-Seetharam, U., et al. 2006. Cell Cycle 5, 873.
Solomon, J.M., et al. 2006. Mol. Cell. Biol. 26, 28.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-January-2009 JSW
SynonymsSilent mating type information regulation 2 homolog, Sirtuin1, GST-Fusion, Human, Recombinant, E. coli
DescriptionRecombinant, human SIRT1 histone deacetylase consisting of amino acids 193-741 fused at the N-terminus to GST and expressed in E. coli. SIRT1 preferentially deacetylates histones H4 at K16 and H3 at K9 and interacts with and deacetylates histone H1 at K26. More than a dozen non-histone proteins have been found to serve as substrates for SIRT1. It plays a role in several biological processes including stress resistance, metabolism, differentiation, and aging. The active deacetylase is useful in the study of SIRT1 regulation and for inhibitor screening.
FormLiquid
FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 0.05% Tween®-20 detergent, 20% glycerol, pH 8.0.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, pH 8.0, 200 µM NAD+, 30 µM fluorescent substrate [Arg-His-Lys-Lys(Ac)], 100 µg/ml BSA, 20 ng/µl SIRT1; incubate at 30°C for 30 min.
Purity≥80% by SDS-PAGE
Specific activity≥8 units/µg protein
Unit definitionOne unit is defined as the amount of enyme required to deacetylate 1 pmol fluorescent peptide substrate [Arg-His-Lys-Lys(Ac)], per min at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesKolthur-Seetharam, U., et al. 2006. Cell Cycle 5, 873.
Solomon, J.M., et al. 2006. Mol. Cell. Biol. 26, 28.