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71136 Rosetta-gami B(DE3) Competent Cells - Novagen

Rosetta-gami B strains combine the key features of BL21, Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71136
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
71136-3 Plastic ampoule 0.4 ml
71136-4 Glass bottle 1 ml
Description
OverviewRosetta-gami B strains combine the key features of BL21 (and its Tuner™ derivative), Origami, and Rosetta to enhance both the expression of eukaryotic proteins and the formation of target protein disulfide bonds in the bacterial cytoplasm. These strains are compatible with ampicillin- or spectinomycin-resistant vectors.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Genotype: F– ompT hsdSB (rB– mB–) gal dcm lacY1 ahpC (DE3) gor522::Tn10 trxB pRARE (CamR, KanR, TetR)


This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number71136
Brand Family Novagen®
References
Product Information
0.4 ml1 mlComponent
•2 × 0.2 ml5 × 0.2 mlRosetta-gami B(DE3) Competent Cells
•2 × 2 ml4 × 2 mlSOC Medium
•10 µl10 µlTest Plasmid
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71136-3 04055977254730
71136-4 04055977254747

Documentation

Rosetta-gami B(DE3) Competent Cells - Novagen SDS

Title

Safety Data Sheet (SDS) 

Rosetta-gami B(DE3) Competent Cells - Novagen Certificates of Analysis

TitleLot Number
71136

Citations

Title
  • Wei Cao, et al. (2007) RAET1E2, a soluble isoform of the UL16 binding protein RAET1E produced by tumor cells, inhibits NKG2D-mediated NK cytotoxicity.. Journal of Biological Chemistry 282, 18922-18928.
  • Christian Zuber, et al. (2007) EDEM1 reveals a quality control vesicular transport pathway out of the endoplasmic reticulum not involving the COPII exit sites. Procedings of the National Academy of Science 104, 4407-4412.
  • Jinghua Chen, et al. (2005) Enzymatic redesigning of biologically active heparan sulfate. Journal of Biological Chemistry 280, 42817-42825.
  • Karsten Mahnke, et al. (2005) Targeting of antigens to activated dendritic cells in vivo cures metastatic melanoma in mice. Cancer Research 65, 7007-7012.
  • John Sergeant, et al. (2005) Composition and architecture of the Schizosaccharomyces pombe rad18 (Smc5-6) complex. Molecular and Cellular Biology 25, 172-184.
  • Atsushi Takabayashi, et al. (2005) Differential use of two cyclic electron flows around photosystem I for driving CO2-concentration mechanism in C4 photosynthesis. Proceedings of the National Academy of Sciences (USA) 102, 16898-16903.
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    User Protocols

    Title
    TB009 Competent Cells
    TB053 Academic and Non-profit Laboratory Assurance Letter