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70953 Rosetta™ Competent Cells - Novagen

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Rosetta™ Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
70953
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
70953-3 Plastic ampoule 0.4 ml
70953-4 Glass bottle 1 ml
Description
OverviewRosetta™ host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters.
Genotype: F- ompT hsdSB(rB- mB-) gal dcm pRARE (CamR)




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.


This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number70953
Brand Family Novagen®
References
Product Information
Components
Guaranteed efficiencyGuaranteed efficiency: > 2 x 10⁶ cfu/µg
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70953-3 04055977256123
70953-4 04055977256130

Documentation

Rosetta™ Competent Cells - Novagen SDS

Title

Safety Data Sheet (SDS) 

Rosetta™ Competent Cells - Novagen Certificates of Analysis

TitleLot Number
70953

Citations

Title
  • Praveen Alamuri and Robert J. Maier. (2006) Methionine sulfoxide reductase in Helicobacter pylori: interaction with methionine-rich proteins and stress-induced expression. Journal of Bacteriology 188, 5839-5850.
  • Carla V. Finkielstein, Michael Overduin and Daniel G. Capelluto. (2006) Cell migration and signaling specificity is determined by Rac1's phosphatidylserine recognition motif. Journal of Biological Chemistry 281, 27317-27326.
  • Sebastian D. Fugmann, et al. (2006) An ancient evolutionary origin of the Rag1/2 gene locus. Proceedings of the National Academy of Sciences (USA) 103, 3728-3733.
  • Jay W. Shin, et al. (2006) Prox1 promotes lineage-specific expression of FGF receptor-3 in lymphatic endothelium: a role for FGF signaling in lymphangiogenesis. Molecular Biology of the Cell 17, 576-584.
  • Natalie Elia, et al. (2005) Excess of Gβe over Gqαe in vivo prevents dark, spontaneous activity of Drosophila photoreceptors. Journal of Cell Biology 171, 517-526.
  • Georgios I. Karras, et al. (2005) The macro domain is an ADP-ribose binding module. European Molecular Biology Organization Journal 24, 1911-1920.
  • Yoshihiro Koshino-Kimura, et al. (2005) Regulation of CAPRICE transcription by MYB proteins for root epidermis differentiation in Arabidopsis. Plant and Cell Physiology 46, 817-826.
  • Lisa Y. Lenertz, et al. (2005) Properties of WNK1 and implications for other family members. Journal of Biological Chemistry 280, 26653-26658.
  • Michail Nomikos, et al. (2005) Role of phospholipase C-ζ domains in Ca2+-dependent phosphatidylinositol 4, 5- bisphosphate hydrolysis and cytoplasmic Ca2+ oscillations. Journal of Biological Chemistry 280, 31011-31018.
  • Stephanie Sentis, et al. (2005) Sumoylation of the estrogen receptor α hinge region regulates its transcriptional activity. Molecular Endocrinology 19, 2671-2684.
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    User Protocols

    Title
    TB009 Competent Cells