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71188 RoboPop™ Ni-NTA His•Bind® Purification Kit

High-throughput, milligram-scale purification of His•Tag® fusion proteins

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information

This product has been discontinued.





The RoboPop™ Ni-NTA His•Bind® Purification Kit is designed for filtration-based 96-well format purification of soluble His•Tag® fusion proteins directly from E. coli cultures without harvesting cells. The kit features PopCulture® Reagent, rLysozyme™ Solution, and Benzonase® Nuclease for centrifuge-free cell lysis and extract preparation in one step. The combination of PopCulture extraction, Ni-NTA His•Bind Resin, and a 2-ml filter plate allows high-throughput processing of up to 5 ml of E. coli culture per well. Whereas the magnetic-based His•Mag™ kit purifies up to 125 µg target protein per 1 ml culture, the filtration-based kit purifies up to 1 mg His•Tag fusion protein per 5 ml culture.

Bacterial culture, cell lysis, and resin binding steps are carried out in standard 24- well plates (not supplied), which accommodate a maximum volume of 5 ml per well. The reaction slurry is then transferred to a 96-well Filter Plate (included) and the washing and elution steps are carried out on a vacuum manifold. The Filter Plate is compatible with standard filter manifolds for manual sample processing, and the entire purification has been validated for robotic sample processing with the Genesis® Freedom™ Workstation from Tecan and the MultiPROBE® II liquid handling work station from PerkinElmer Life Sciences. A 96-well Collection Plate (1-ml wells) with an air-tight aluminum foil sealer is provided for storage of the purified proteins.

Description

Catalogue Number71188 Brand Family Novagen®
Features and benefits
  • High-throughput, 96-well protein purification directly from E. coli without harvesting cells
  • Centrifuge-free cell lysis and extract preparation in one step
  • Large-scale culture processing—up to 5 ml per well
  • High protein yield—up to 1 mg per well
  • Validated for robotic sample processing with the Genesis® Freedom™ Workstation from Tecan and the Packard-brand MultiPROBE® II liquid handling work station from PerkinElmer Life Sciences
References
ReferencesGrabski, A., et al. 2002. inNovations 14, 2.
Product Information
•75 mlPopCulture® Reagent
•25 mlNi-NTA His•Bind Resin
•125 ml4X Ni-NTA Bind Buffer
•2 x 125 ml4X Ni-NTA Wash Buffer
•50 ml4X Ni-NTA Elute Buffer
•12-ml 96-well Filter Plate
•11-ml 96-well Collection Plate
with Sealer
•300 KUrLysozyme™ Solution
(Note: 1 KU = 1000 units)
•1 mlrLysozyme Dilution Buffer
•10,000 UBenzonase® Nuclease,
Purity > 90%
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage Multiple storage requirements
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71188 0