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504043 Rho Inhibitor II, Y16 - CAS 429653-73-6 - Calbiochem

504043
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Overview

Key Spec Table

CAS #Empirical Formula
429653-73-6C₂₄H₂₀N₂O₃

Products

Catalogue NumberPackaging Qty/Pack
5.04043.0001 Glass bottle 10 mg
Description
OverviewA cell-permeable pyrazolidinedione compound that is shown to target RhoGEF DH-PH domain junction with high affinity (Kd = 65 nM) and effetively prevent RhoGEFs LARG, p115, and PDZ from interacting with RhoA, while displaying little potency against DBL-RhoA, LBC-RhoA, intersectin-Cdc42, or TrioN-Cdc42 interaction. Shown to completely prevent serum-induced activation of cellular RhoA, but not Cdc42 or Rac1, in NIH-3T3 cultures (10 µM) and RhoA downstream signaling events. Greatly synergizes with Rho GEF-binding domain blocker Rhosin (Cat. No. 555460) in blocking RhoA-LARG interaction and in preventing cellular RhoA activation (both drug at 5 µM).
Catalogue Number504043
Brand Family Calbiochem®
SynonymsLARG Rho-Binding Domain Blocker, (E)-4-(3-(3-Methylbenzyloxy)benzylidene)-1-phenylpyrazolidine-3,5-dione, (4E)-4-(3-(3-Methylbenzyloxy)benzylidene)-1-phenyl-3,5-pyrazolidinedione, p115 Rho-Binding Domain Blocker, PDZ Rho-Binding Domain Blocker
References
ReferencesShang, X., et al. 2013. Proc. Natl. Acad. Sci. USA. 110, 3155.
Product Information
CAS number429653-73-6
FormOrange powder
Hill FormulaC₂₄H₂₀N₂O₃
Chemical formulaC₂₄H₂₀N₂O₃
ReversibleY
Structure formula ImageStructure formula Image
Quality LevelMQ100
Biological Information
Primary TargetRhoGEF
Purity≥95% by HPLC
Physicochemical Information
Cell permeableY
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze -20°C. Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Global Trade Item Number
Catalogue Number GTIN
5.04043.0001 04055977264258