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14-325 Rac/cdc42 Assay Reagent (PAK1 PBD, agarose), 300 µg

14-325
300 µg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue Number14-325
Brand Family Upstate
Trade Name
  • Upstate
DescriptionRac/cdc42 Assay Reagent (PAK1 PBD, agarose), 300 µg
OverviewGST fusion-protein, corresponding to the p21-binding domain (PBD, residues 67-150) of human PAK-1 provided bound to glutathione agarose.

Specifically binds to and precipitates Rac-GTP and cdc42-GTP from cell lysates.
Product Information
Quality LevelMQ100
Applications
ApplicationSpecifically binds to & precipitates Rac-GTP & cdc42-GTP from cell lysates. For use in Affinity Binding Assays.
Key Applications
  • Affinity Binding Assay
Biological Information
SourceHuman PAK-1, expressed in E. coli.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
Gene Symbol
  • CDC42
  • CDC42Hs
  • G25K
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P60953 # Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Causes the formation of thin, actin-rich surface projections called filopodia.
SIZE: 191 amino acids; 21311 Da
SUBUNIT: The GTP-bound form interacts with CCPG1 (By similarity). Interacts with CDC42EP1, CDC42EP2, CDC42EP3, CDC42EP4, CDC42EP5, PARD6A, PARD6B and PARD6G in a GTP-dependent manner. Interacts with activated CSPG4 and with BAIAP2. Interacts with Zizimin1/DOCK9 and Zizimin2/DOCK11, which activate it by exchanging GDP for GTP. Interacts with NET1 and TCGAP/SNX26. Part of a complex with PARD3, PARD6A or PARD6B and PRKCI or PRKCZ.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential).
SIMILARITY: SwissProt: P60953 ## Belongs to the small GTPase superfamily. Rho family. CDC42 subfamily.
Product Usage Statements
Quality AssuranceRoutinely evaluated by Affinity Precipitation/Immunoblot Analysis
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -20°C
Packaging Information
Material Size300 µg
Global Trade Item Number
Catalogue Number GTIN
14-325 04053252588532