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SCR066 Quantitative Alkaline Phosphatase ES Characterization Kit

This Quantitative Alkaline Phosphatase Embryonic Stem (ES) Characterization Kit easily quantifies the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
SCR066
100 assays  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCR066
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionQuantitative Alkaline Phosphatase ES Characterization Kit
OverviewMillipore’s Quantitative Alkaline Phosphatase ES Characterization Kit allows researchers to easily quantify the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay. This kit can be used in conjunction with Millipore’s Alkaline Phosphatase Detection kit (Cat. No. SCR004) to fully characterize the levels of alkaline phosphatase present within an embryonic stem cell culture.
Background InformationEmbryonic stem (ES) cells are pluripotent cells derived from the inner cell mass (ICM) of preimplantation embryos and are capable of unlimited, undifferentiated proliferation in vitro under appropriate cell culture conditions. Undifferentiated embryonic stem cells are characterized by high expression levels of stage-specific embryonic antigens (SSEA-1, 3 and 4), tumor rejection antigens (TRA-1-60 and TRA-1-81), OCT-4 and alkaline phosphatase. These undifferentiated markers are downregulated upon spontaneous or induced differentiation.

Alkaline phosphatase (ALP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. The enzyme is present within all tissues of the body but is elevated in cells of the liver, kidney, bone, placenta, embryo and under specific disease states. Under alkaline conditions (pH>10), ALP can catalyze the hydrolysis of p-nitrophenylphosphate (p-NPP) into phosphate and p-nitrophenol, a yellow colored by-product of the catalytic reaction. The amount of p-nitrophenol produced is proportional to the amount of alkaline phosphatase present within the reaction. The amount of ALP can thus be reliably quantified by reading the amount of p-nitrophenol amassed after the catalytic reaction at 405 nm on a spectrophotometer.
References
Product Information
Components
  • p-NPP Substrate Concentrate (50X): (Part No. ES008-200UL) 200 μL
  • p-NPP Buffer: (Part No. ES011-10ML) 10 mL
  • Reaction Stop Solution: (Part No.CS200653) 5 mL
  • 1X Wash Solution: (Part No. CS200652) 2 X 125 mL
  • Recombinant Alkaline Phosphatase Standard: (Part No. CS200654) 0.5 μg (10 μg/mL)
HS Code3822 19 90
Quality LevelMQ100
Applications
ApplicationThis Quantitative Alkaline Phosphatase Embryonic Stem (ES) Characterization Kit easily quantifies the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay.
Key Applications
  • Stem Cell Culture
Biological Information
Species Reactivity
  • Mammals
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Human Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Cardiac Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsAll reagents are to be stored at 2∘ to 8∘C until expiration date. p-NPP substrate concentrate and p-NPP buffer are light sensitive and should not be exposed to light. Reaction stop solution contains 1N sodium hydroxide and is corrosive. Avoid skin contact and inhalation.
Packaging Information
Material Size100 assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR066 04053252660665

Documentation

Quantitative Alkaline Phosphatase ES Characterization Kit SDS

Title

Safety Data Sheet (SDS) 

Quantitative Alkaline Phosphatase ES Characterization Kit Certificates of Analysis

TitleLot Number
Quantitative Alkaline Phosphatase ES Characterizat 2470793
Quantitative Alkaline Phosphatase ES Characterization Kit 3067729
Quantitative Alkaline Phosphatase ES Characterization Kit 2866784
Quantitative Alkaline Phosphatase ES Characterization Kit 2927699
Quantitative Alkaline Phosphatase ES Characterization Kit 2954499
Quantitative Alkaline Phosphatase ES Characterization Kit 3107273
Quantitative Alkaline Phosphatase ES Characterization Kit 3082340
Quantitative Alkaline Phosphatase ES Characterization Kit 3006579
Quantitative Alkaline Phosphatase ES Characterization Kit -
Quantitative Alkaline Phosphatase ES Characterization Kit - 2325087 2325087
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Technical Info

Title
Millipore Tools for Characterizing Induced Pluripotent Stem Cells

Data Sheet

Title
Quantitative Alkaline Phosphatase ES Characterization Kit