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14-1058 PvuRts1I (5-hmC Restriction Endonuclease)

PvuRts1I is an enzyme that can specifically digest DNA containing 5-hydroxymethylcytosine (5-hmC). This restriction endonucleaseis used to analyze 5-hmC patterns genomic DNA by cleaving both glucosylated & non-glucosylated 5-hmC DNA sequences.

PvuRts1I (5-hmC Restriction Endonuclease) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
14-1058
200 Units  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key Applications
Enzyme Assays
Description
Catalogue Number14-1058
DescriptionPvuRts1I (5-hmC Restriction Endonuclease)
OverviewSpecifications
  • Enzme Recognition Site: hmCN11-12/N910G
  • Unit Definition: 1 Unit will digest 1µg of hmC-containing DNA to completion in 20 minutes at room temperature in 1X reaction buffer.
  • Purity >95% purity by SDS PAGE
  • Source: Proteus vulgaris
  • Host: E. coli
Alternate Names
  • PvuRts
  • Pvu
Background InformationPvuRts1I is an enzyme isolated from the gram negative bacterium Proteus vulgaris that can specifically digest DNA containing 5-hmC (Szwagierczak et al., Nucleic Acids Research, 2011) while leaving cytosine and 5-mC intact. This endonuclease can be used to analyze 5-hmC patterns in mammalian genomes by cleave cutting both glucosylated and non-glucosylated 5-hmC containing DNA sequences.

Research studies of DNA methylation and gene expression support the presence of 5-hydroxymethylcytosine (5-hmC) as a sixth base in mammalian genomes. 5-hmC is generated by Ten Eleven Translocation (TET) family of enzymes through oxidation of 5-methylcytosine (5-mC). Chemically, 5-hmC differs from 5-mC by the presence of a hydroxyl on the methyl group at position 5 of cytosine.
References
Product Information
PresentationMaterials Provided:
PvuRts1I 5-hmC Endonuclease: 100U (1U/µL) in a buffer containing 20mM Tris pH 8.0, 150mM NaCl, 1mM DTT and 50% Glycerol.

PvuRts1I 5-hmC 10X Reaction Buffer: 500µL of buffer containing 200mM Tris pH 8.0, 1.5M NaCl, 50mM MgCl2 and 10mM DTT.
Quality LevelMQ100
Applications
ApplicationPvuRts1I is an enzyme that can specifically digest DNA containing 5-hydroxymethylcytosine (5-hmC). This restriction endonucleaseis used to analyze 5-hmC patterns genomic DNA by cleaving both glucosylated & non-glucosylated 5-hmC DNA sequences.
Key Applications
  • Enzyme Assays
Biological Information
HostE. coli
PurityGreater than 95% purity by SDS PAGE
Molecular Weight35 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable at -20ºC for 1 year from date of receipt.
Packaging Information
Material Size200 Units
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-1058 04053252941047

Documentation

PvuRts1I (5-hmC Restriction Endonuclease) Certificates of Analysis

TitleLot Number
PvuRts1I - QVP1310031 QVP1310031
PvuRts1I - VP2001100 VP2001100
PvuRts1I - VP2210040 VP2210040
PvuRts1I - VP2410070 VP2410070
PvuRts1I -VP1506072 VP1506072
PvuRts1I 5-hmC Endonuclease VP1701072
PvuRts1I 5-hmC Endonuclease - VP2203092 VP2203092

References

Reference overviewPub Med ID
Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine.
Szwagierczak, Aleksandra, et al.
Nucleic Acids Res., 39: 5149-56 (2011)  2011

Show Abstract 		21378122 21378122

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