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17-10082 PureEpi™ Chromatin Preparation and Optimization Kit

The PureEpi Chromatin Preparation & Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function
17-10082
12 chromatin preparations  Kit capacity: 12 chromatin preparations
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number17-10082
Brand Family Upstate
Trade Name
  • PureEpi
  • Upstate
DescriptionPureEpi™ Chromatin Preparation and Optimization Kit
OverviewThe PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results.

Features & Benefits
  • Complete set of materials for up to 12 chromatin preparations
  • Optimized streamlined protocol with only a single buffer for sonication, IP, or wash
  • Protocols for using cells or tissues
  • Compatible with Magna ChIP HiSens and EZ-Magna ChIP HiSens chromatin immunoprecipitation kits.
  • Compatible with ChIPAb+ validated antibody and primer sets
Materials Required but Not DeliveredReagents
  • Cells, stimulated or treated as desired
  • Tissue culture supplies appropriate for your cultured cell experiment
  • 1 X PBS
  • 37% Formaldehyde

The following items are necessary for the recommended method of crosslink reversal and DNA purification of sample:
  • Phenol
  • Chloroform:Isoamyl Alcohol
  • Phase Lock tubes
  • 5M NaCl
  • Glycogen (20 mg/mL)
  • 100% Ethanol
  • 70% Ethanol
  • DNA purification column may possibly be used to purify DNA if organic extraction is not possible
  • RNAse A (10 mg/mL)
  • Proteinase K (10 mg/mL)
  • TE Buffer (Nuclease Free)
  • DNase and RNase-free sterile H2O
  • Liquid Nitrogen
  • 100 bp DNA Ladder
  • 1kb DNA Ladder
  • Agarose gel
  • DNA 12000 reagents for BioAnalyzer (Agilent)


Equipment
  • Vortex mixer
  • Microfuge
  • Sonicator
  • Variable temperature water bath or incubator
  • Timer
  • Variable volume (5-1000 µL) pipettors + tips
  • Cell scraper
  • 1.5 mL microfuge tubes
  • NanoDrop® (Thermo-Fisher) or equivalent small volume capable spectrophotometer
  • Dewar flask for liquid nitrogen
  • Microscope and cell counter (Scepter or hemocytometer)
  • BioAnalyzer (Agilent)
References
Product Information
Components
  • 10X Glycine
  • Nuclei Isolation Buffer
  • SCW Buffer (Sonication/ChIP/Wash)
  • Crosslink Reversal Buffer
  • Protease Inhibitor Cocktail II
PresentationTwo boxes containing all necessary reagents to perform 12 chromatin preparations using 15 cm plates of cultured cells or tissue samples.
Quality LevelMQ100
Applications
ApplicationThe PureEpi Chromatin Preparation & Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
Packaging Information
Material Size12 chromatin preparations
Material PackageKit capacity: 12 chromatin preparations
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
17-10082 04053252855375