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71772 ProteoExtract® Transmembrane Protein Extraction Kit

71772
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
71772-3 20 rxn
Description
OverviewThe ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
Catalogue Number71772
Brand Family Novagen®
References
ReferencesNakamura, E., et al. 2008. Cancer Sci. 99, 1390.
Product Information
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 25-33

Toxic if swallowed.
Danger of cumulative effects.
S PhraseS: 37-45

Wear suitable gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Toxic
Storage +2°C to +8°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71772-3 04055977250909

Documentation

ProteoExtract® Transmembrane Protein Extraction Kit Certificates of Analysis

TitleLot Number
71772

References

Reference overview
Nakamura, E., et al. 2008. Cancer Sci. 99, 1390.

Citations

Title
  • Waetzig G.H., et al. 2010. The Journal of Biological Chemistry 285, 1781.
  • Ernst, C.M., et al. 2009. doi: 10.1371/journal.ppat.1000660
  • User Protocols

    Title
    TB477