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203195 Protein A, Biotin Conjugate

This Protein A, Biotin Conjugate is validated for use in Solid Phase Immunoassay.

Protein A, Biotin Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
203195
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
203195-2MG Plastic ampoule 2 mg
Description
OverviewNative protein A conjugated to biotin.
Catalogue Number203195
Brand Family Calbiochem®
References
Product Information
FormLiquid
FormulationIn 10 mM PBS, 1% BSA, pH 7.6.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application NotesSolid Phase Immunoassay (1:10,000)
Application CommentsThe protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
PurityA single band at MW 50,000 by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
203195-2MG 04055977221114

Documentation

Protein A, Biotin Conjugate SDS

Title

Safety Data Sheet (SDS) 

Protein A, Biotin Conjugate Certificates of Analysis

TitleLot Number
203195
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-June-2007 RFH
ApplicationSolid Phase Immunoassay (1:10,000)
DescriptionNative protein from Straphylococcus Aureus, conjugated to biotin. Recognizes the Fc portions of immunoglobulins of various species.
BackgroundLong-chain NHS-Biotin-labeled Protein A is an extremely useful reagent because of its high binding affinity for avidin, streptavidin and avidin- or streptavidin-labeled compounds. Protein A is the universal secondary antibody because it binds to the Fc portions of most immunoglobulin classes. It is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. It is especially useful in immunohistochemical studies because, unlike secondary antibodies, it does not bind to cell Fc receptors, and therefore will not give high background reactions.
FormLiquid
FormulationIn 10 mM PBS, 1% BSA, pH 7.6.
Concentration Label Please refer to vial label for lot-specific concentration
PurityA single band at MW 50,000 by SDS-PAGE
Preservative≤0.1% sodium azide
CommentsThe protein A: biotin molar ratio is determined on a lot-specific basis. Binding strength is as follows: strongly reactive, guinea pig, human IgG1, IgG2, and IgG4, and rabbit; weakly reactive: bovine, human IgA, IgM, and IgE, and mouse; nonreactive: goat, horse, human IgD and IgG3, rat, and sheep. o-phenylenediamine, 1.0 mg/ml in citrate buffer, pH 4.5, 0.1% urea peroxide at 25°C, was used as substrate for testing biotin-Protein A and avidin peroxidase functionality. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling