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71113 PopCulture® GST•Mag™ Purification Kit

PopCulture extraction and GST•Tag™ fusion protein purification from E. coli cultures

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71113
  
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Overview

Replacement Information

This product has been discontinued.





The PopCulture® GST•Mag™ Purification Kit is designed for purification of GST•Tag™ fusion proteins directly from E. coli cultures without harvesting cells. The procedure combines PopCulture total culture extraction with magnetic affinity purification using GST•Mag Agarose Beads.

PopCulture Reagent is a detergent-based concentrate that can be added directly to cultures of E. coli to effectively extract proteins without the need for centrifugation. Use of GST•Mag Agarose Beads enables the entire procedure to be carried out in a single tube without using columns or centrifugation.

The PopCulture GST•Mag Purification Kit combines PopCulture Reagent, GST•Mag Agarose Beads, corresponding buffers and rLysozyme™ Solution. This kit enables processing of 40 × 3-ml cultures with yields up to 150 µg GST•Tag fusion protein per 3-ml culture, based on bead binding capacity. The kit is compatible with the Novagen Magnetight™ Separation Stand. For 96-well processing using PopCulture, see our various RoboPop™ Purification Kits.

Description


Catalogue Number71113 Brand Family Novagen®
Features and benefits
  • Binding capacity of up to 2 mg/ml
  • Glutathione-derivatized magnetic agarose form with 3 µm average bead size
  • No need to separate cells from culture media: use 25 µl settled beads per milliliter culture (50 µl 50% v/v suspension)
  • No need to mechanically disrupt cells
  • No need to clarify cell extracts prior to purification
  • Direct affinity adsorption of target proteins to resin from the total culture extract
  • Ability to rapidly perform the entire cell growth and purification process in a single tube or well
References
Product Information
•15 mlPopCulture Reagent
•3 × 1 mlGST•Mag Agarose Beads
•2 × 100 ml10X GST Bind/Wash Buffer
•40 ml10X Glutathione Reconstitution Buffer
•1 gGlutathione, Reduced
•300 KUrLysozyme Solution
•1 mlrLysozyme Dilution Buffer
DeclarationThis product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
71113 0

Documentation

PopCulture® GST•Mag™ Purification Kit Certificates of Analysis

TitleLot Number
71113

User Protocols

Title
TB235 GST•Bind™ Kits