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CC321-2 PhotoCol®-LAP, Methacrylated Collagen Hydrogel Kit

Photocrosslinked type I collagen hydrogel used for 3D cell culture and as a bioink for bioprinting (CC321 Kit Component 2)

CC321-2
1 Kit  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberCC321-2
DescriptionPhotoCol®-LAP, Methacrylated Collagen Hydrogel Kit
Overview3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks.

The PhotoCol®-LAP, Methacrylated Collagen Hydrogel Kit (CC321: CC321-1, CC321-2) is based upon purified type I bovine collagen methacrylate (ColMA), which when photocrosslinked provides a native-like 3D environment for cells. In addition to type I bovine collagen methacrylate, the kit includes the photoinitiator lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) and other reagents for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds). The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen that has been modified by reacting with the protein’s free amines, primarily the ɛ-amines groups of the lysine residues as well as the α-amines groups on the N-termini. > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content that was isolated from a closed herd and purified using controlled manufacturing processes.

References

1) Isaacson, A, Swioklo, S. & Connon, C. J. 3D bioprinting of a corneal stroma equivalent. Experimental Eye Research 173, 188–193 (2018).
2) Maloney, E, et al. Immersion Bioprinting of Tumor Organoids in Multi-Well Plates for Increasing Chemotherapy Screening Throughput. Micromachines 11, 208 (2020).
3) Lelièvre, S, et al. Cell Culture and Coculture for Oncological Research in Appropriate Microenvironments. Current Protocols Chem Biol. 2019 Jun;11(2):e65.

PhotoCol® is a registered trademark of Advanced BioMatrix, Inc.
Alternate Names
  • Collagen Methacrylate
  • ColMA
  • Collagen Bioink
  • Collagen Hydrogel
References
Product Information
Components
  • CC321-2 (Store at 2-8°C): Methacrylated Collagen, 1 X 100 mg (CS226428), 20 mM Acetic Acid, 1 X 50 mL (CS226413), LAP Photoinitiator, 1 X 100 mg (CS226412).
  • Note: The complete PhotoCol®-LAP, Methacrylated Collagen Hydrogel Kit (CC321) consists of CC321-1 and CC321-2 sold seperately.
Applications
ApplicationPhotocrosslinked type I collagen hydrogel used for 3D cell culture and as a bioink for bioprinting (CC321 Kit Component 2)
Key Applications
  • Cell Culture
Application NotesPhotocrosslinking is initiated using a 405 nm blue light source.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceSterility (USP modified): No Growth
Endotoxin LAL: ≤ 10.0 EU/ml
Gel Formation: ≤ 40 min
Kinetic Gel Test: ≤ 40 min
Purity (SDS Page Silver Stain): ≥ 99%
Degree of Methacrylation: ≥ 20%
pH: 3.0-4.0
Osmolality: ≤ 40 mOsm/Kg H20
Young’s Modulus: Characteristic
Electrophoretic Pattern: Characteristic
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 Kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CC321-2 04061842348060