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525189 Phospholipase C-γ-2, His•Tag®, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Phospholipase C-γ-2, Human, Recombinant

525189
  
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Overview

Replacement Information
Description
Overview

This product has been discontinued.



Full-length, recombinant, human phospholipase C γ-2 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. Production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes, which are crucial enzymes in transmembrane signaling. PLC-γ-2 is phosphorylated on tyrosine residues following ligand-induced activation of a variety of growth factor receptors and immune system receptors, resulting in increased phospholipase activity. The full-length proteins contains the following domains: C2 domain (aa 1059-1152); PH domain (aa 20-131); PI-PLC X-box catalytic domain (aa 312-456); PI-PLC Y-box catalytic domain (aa 930-1044); two SH2 domains (aa 532-635, aa 646-735); and a SH3 domain (aa 769-829). It has been reported to be phosphorylated at Tyr753 and Tyr759 (Btk, Src, Fyn, Lck), and Tyr1217 and Tyr1245 by Bcr-Abl.
Catalogue Number525189
Brand Family Calbiochem®
SynonymsPhospholipase C-γ-2, Human, Recombinant
Application Data
References
ReferencesRodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
Lennon, G., et al. 1996. Genomics 33, 151.
Product Information
FormLiquid
FormulationIn PBS.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
525189 0

Documentation

Phospholipase C-γ-2, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
525189

References

Reference overview
Rodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
Lennon, G., et al. 1996. Genomics 33, 151.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-August-2008 RFH
SynonymsPhospholipase C-γ-2, Human, Recombinant
Application Data
DescriptionFull-length, recombinant, human phospholipase C γ-2 fused at the N-terminus to His•Tag® and S•Tag™ sequences and expressed in S. frugiperda insect cells. This preparation is qualified for use as a substrate for protein tyrosine kinases in in vitro assays. Intrinsic enzyme activity has not been tested. Production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes, which are crucial enzymes in transmembrane signaling. PLC-γ-2 is phosphorylated on tyrosine residues following ligand-induced activation of a variety of growth factor receptors and immune system receptors, resulting in increased phospholipase activity. The full-length proteins contains the following domains: C2 domain (aa 1059-1152); PH domain (aa 20-131); PI-PLC X-box catalytic domain (aa 312-456); PI-PLC Y-box catalytic domain (aa 930-1044); two SH2 domains (aa 532-635, aa 646-735); and a SH3 domain (aa 769-829). It has been reported to be phosphorylated at Tyr753 and Tyr759 (Btk, Src, Fyn, Lck), and Tyr1217 and Tyr1245 by Bcr-Abl.
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Irritant
ReferencesRodriguez, R., et al. 2001. J. Biol. Chem. 276, 47982.
Lennon, G., et al. 1996. Genomics 33, 151.