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524746 Phosphodiesterase 4D5, Active, Human, Recombinant, S. frugiperda

524746
  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Overview

This product has been discontinued.





Full-length, recombinant, human phosphodiesterdase 4D5 (PDE4D5) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE4D5 is expressed primarily in cardiac and vascular myocytes where it is found preferentially sequestered to β-arrestin. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
Catalogue Number524746
Brand Family Calbiochem®
SynonymscAMP-specific phosphodiesterase 4D isoform 5, PDE4D5
Application Data
Increasing concentration of PDE inhibitor, rolipram, was added to enzyme and the activity assayed.
References
ReferencesBaillie., et al. 2007. Biochem J. 404, 71.
Lynch, M.J., et al. 2005. J. Biol. Chem. 280,33178.
Bolger, G.B., et al. 2002. BMC Biochem. 3, 24.


Product Information
Activity≥ 0.5 U/µl
Unit of DefinitionOne unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5 at 30°C.
FormLiquid
Formulation150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
524746 0

Documentation

Phosphodiesterase 4D5, Active, Human, Recombinant, S. frugiperda Certificates of Analysis

TitleLot Number
524746

References

Reference overview
Baillie., et al. 2007. Biochem J. 404, 71.
Lynch, M.J., et al. 2005. J. Biol. Chem. 280,33178.
Bolger, G.B., et al. 2002. BMC Biochem. 3, 24.


Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-March-2009 JSW
SynonymscAMP-specific phosphodiesterase 4D isoform 5, PDE4D5
Application Data
Increasing concentration of PDE inhibitor, rolipram, was added to enzyme and the activity assayed.
DescriptionFull-length, recombinant, human phosphodiesterdase 4D5 (PDE4D5) expressed in S. frugiperda insect cells using a baculovirus expression system. PDE4D5 is expressed primarily in cardiac and vascular myocytes where it is found preferentially sequestered to β-arrestin. Supplied as a partially purified preparation of catalytically active enzyme useful for inhibitor and activator assays.
FormLiquid
Formulation150 mM NaCl, 10 mM Tris, 0.1% NP-40, pH 8.3
Activity≥ 0.5 U/µl
Unit definitionOne unit is defined as the amount of enzyme that is required to convert 1 pmol cAMP to AMP per min at pH 7.5 at 30°C.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesBaillie., et al. 2007. Biochem J. 404, 71.
Lynch, M.J., et al. 2005. J. Biol. Chem. 280,33178.
Bolger, G.B., et al. 2002. BMC Biochem. 3, 24.