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524737 Phosphodiesterase 4C2, His•Tag®, Human, Recombinant, S. frugiperda

524737
  
Purchase on Sigma-Aldrich

Overview

Description
Overview

This product has been discontinued.





Full-length, recombinant, human phosphodiesterdase 4C2 (accession no. U88712) fused at the N-terminus to a His•Tag® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation of the catalytically active enzyme.
Catalogue Number524737
Brand Family Calbiochem®
SynonymsPDE4C2, cAMP-Specific Phosphodiesterase 4C2
Application Data
Phosphodiesterase 4C2 was treated with increasing concentration of the inhibitor, rolipram, and the activity assayed. the IC50 for this inhibition is 7.87 µM.
References
ReferencesGibson, L.C.D., et al. 2006. Eur. J. Pharmacol. 538, 39.
Sullivan, M., et al. 1999. Cell Signal. 11, 735.
Engels, P., et al. 1995. FEBS Lett. 358, 305.
Product Information
Activity≥ 0.5 units/µl
Unit of DefinitionOne unit is defined as the amount of enzyme that will convert 1 pmol cAMP to AMP per min at 30°C, pH 7.5.
FormLiquid
FormulationIn 150 mM NaCl, 10 mM Tris-HCl, 0.1% NP-40, pH 8.3.
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
524737 0